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BACKGROUND: Chicken meat has become a major source of protein for human consumption. However, the quality of the meat is not yet under control, especially since pH values that are too low or too high are often observed. In an attempt to get a better understanding of the genetic a...

2022-06-01 | MTBLS2970 | MetaboLights
Cattle are often fed high concentrate diets to increase energy intake and improve overall animal performance. Such diets also cause changes in fermentation patterns and epithelial function. However, the molecular mechanisms involved in regulating epithelial function for cattle fed high concentrate...
ORGANISM(S): Bos taurus 
Analysis of the transcriptomes of cell grown at different pH levels pH6.0, pH7.5, pH9.0; Cells were grown in a fermenter at different pH values to an oD600 between 4.5 to 11.5
ORGANISM(S): Corynebacterium glutamicum 

Circadian clocks coordinate mammalian behaviour and physiology enabling organisms to anticipate 24-hour cycles. Transcription-translation feedback loops are thought to drive these clocks in most of mammalian cells. However, red blood cells (RBCs), which do not contain a nucleus, and cannot perfor...

2020-11-13 | MTBLS1285 | MetaboLights
In bottom-up proteomics, the complexity of proteome profiling is usually limited by the number of identified proteins in nanoflow LC MS/MS analysis. Herein, we developed a fully automatic online 2D nanoLC MS/MS system using both high-pH and low-pH RPLCs for a comprehensive proteomics analysis.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-06-05 | MSV000089609 | MassIVE
The change in pH of the mobile phase drastically changes the retention mechanism of peptides, rendering currently available retention time prediction models useless. The aim of this study is to create a dataset of peptide retention times under basic (pH 9) HPLC conditions, allowing for the creation ...
ORGANISM(S): Homo sapiens (Human) 
2026-02-13 | PXD064311 | Pride
The objective of the study was to identify the fertility-associated metabolites in bovine spermatozoa using liquid chromatography-mass spectrometry (LC-MS). Six Holstein Friesian crossbred bulls (three high-fertile and three low-fertile bulls) were the experimental animals. Sperm proteins were isola...
2021-12-07 | MTBLS1071 | MetaboLights
Relative protein abundances of Escherichia coli growing exponentially on minimal medium with acetate or glucose as the sole carbon source were investigated in a quantitative shotgun proteome analysis with TMT6-plex isobaric tags. Peptides were separated by high resolution high/low pH 2D-LC, using an...
ORGANISM(S): Escherichia coli 
2016-10-11 | PXD003863 | Pride
Cattle are often fed high concentrate diets to increase energy intake and improve overall animal performance. Such diets also cause changes in fermentation patterns and epithelial function. However, the molecular mechanisms involved in regulating epithelial function for cattle fed high concentrate d...
ORGANISM(S): Bos taurus 
2010-10-02 | GSE19802 | GEO
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