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ProteomicsPhosphoproteomics sample preparation impacts biological interpretation of signalling responses
Quantitative data-dependent acquisition phosphoproteomics (DDAP) has revolutionised cell signaling studies. However, the impact of the specific DDAP sample preparation method on the phosphoproteome quantified is unclear as each study quantifies only a fraction of the phosphoproteome. Here, we compar...
ORGANISM(S): Mus musculus (Mouse) 
2021-12-25 | PXD025376 | Pride
Phosphoproteomics Tio2 Mouse High-throughput phosphoproteomics Scxphos Sample preparation Highphos Comparative analysis 
ProteomicsHigh-throughput phosphoproteomics reveals in vivo insulin signaling dynamics
In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and p...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-06-25 | MSV000082502 | MassIVE
ProteomicsProteomics, phosphoproteomics and miRNA analysis of circulating extracellular vesicles through automated and high-throughput isolation
Here, we introduce a magnetic bead-based EV enrichment approach (EVrich) for automated and high-throughput processing of urine samples. Parallel enrichments can be performed in 96-well plates for downstream cargo analysis, including EV characterization, miRNA, proteomics and phosphoproteomics analys...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD033595 | Pride
Mirna Mass spectrometry Prostate cancer Extracellular vesicles Phosphoproteomics 
ProteomicsHigh-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform
Mass spectrometry has transformed the field of cell signalling by enabling global studies of dynamic protein phosphorylation (‘phosphoproteomics’). Recent developments are enabling increasingly sophisticated phosphoproteomics studies, but practical challenges remain. The EasyPhos workflow addres...
ORGANISM(S): Homo sapiens (Human) 
2018-09-07 | PXD009227 | Pride
Glioblastoma Cancer Proteomics Post-translational modification Signal transduction Mass spectrometry Phosphorylation Egf Phosphoproteomics 
ProteomicsHigh-Throughput Proteomics Sample Preparation Using 96-Channel Pipettor and Magnetic Pin Device
The advent of high-throughput proteomics has created an urgent need for parallel increases in sample processing productivity. However, workflows—particularly those involving PTM profiling—remain highly complex and consist of multiple steps that demand exceptional reproducibility. Many laboratories a...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2026-02-17 | PXD069607 | Pride
Oasis hlb Proteomics Sp3 Phosphoproteomics Pac High-throughput samples preparation 
ProteomicsHigh-throughput phosphoproteomics reveals in vivo insulin signaling dynamics
In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and p...
ORGANISM(S): Mus musculus (Mouse) 
2015-08-19 | PXD001792 | Pride
Mouse Brain Kidney Q exactive Liver Phosphoproteome 
UnknownProteomics, Phosphoproteomics and Mirna Analysis of Circulating Extracellular Vesicles through Automated and High-Throughput Isolation.
Not available
S-EPMC9265938 | biostudies-literature
OtherHigh-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.
Data from ProteomeXchange, PXD ID: PXD001792. File: 2_QEp2_LC11_SeHu_LivPhos_Insulin_10m_Bio3.mzml. Published as part of Nat Biotechnol. 2015 Aug 17 . From the Abstract: {{i}} Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-t...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32320017798 | GPMDB
Resource Reanalysis 
ProteomicsHeLa phosphoproteomics - TiO2-enriched phosphopeptides
TiO2-enriched phosphopeptides
ORGANISM(S): Homo sapiens (Human) 
2011-08-08 | PRD000526 | Pride
ProteomicsTIHAMAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics Analysis
Protein post-translational modifications (PTMs) are pivotal molecular events in plant cells, encompassing processes such as protein folding and enzyme activation. Among these PTMs, N-glycosylation and phosphorylation hold particular importance, as they play essential roles in regulating plant growth...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-04-03 | PXD047065 | Pride
N-glycoproteomics Hilic Arabidopsis thaliana Imac Aba signaling Phosphoproteomics 
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