High-molecular-weight glutenin subunits (HMW-GS) play an essential role in the end-use quality of wheat (Triticum aestivum L.). We developed a targeted liquid chromatography-tandem mass spectrometry method in parallel reaction monitoring (LC-MS/MS-PRM) to detect and differentiate wheat HMW-GS in Ger...
Transgenic cadenza lines express five HMW subunits encoded by:Glu-B1 (14, 15) and Glu D1 (5, 10) and transgene Glu-A1 (Ax1). Transcriptomes of the wheat line B1118-8-4(6) (produced by co transformation with two plasmids: one carried the transgene HMW-GS 1Ax1and other the bar and gus gene sequence...