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TranscriptomicsTranscription profiling of Streptomyces coelicolor wild type and phP-null mutants at different time points during phosphate starvation
S. coelicolor was grown in low-phosphate concentration R5 liquid medium which facilitated the transition to a phosphate starvation induced stationary phase and the subsequent induction of the PhoP regulon. Global gene expression profiling of a wild-type and a phoP-null mutant was performed using sam...
ORGANISM(S): Streptomyces coelicolor 
2012-08-30 | E-MAXD-51 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Streptomyces coelicolor wild type and phP-null mutants during phosphate starvation to determine binding locations of PhoP
S. coelicolor was grown in low-phosphate concentration R5 liquid medium which facilitated the transition to a phosphate starvation induced stationary phase and the subsequent induction of the PhoP regulon. The genome wide binding locations of PhoP during phosphate starvation were determined by chrom...
ORGANISM(S): Streptomyces coelicolor 
2012-08-30 | E-MAXD-50 | biostudies-arrayexpress
TranscriptomicsDifferential expression of S. coelicolor M145 vs. an abrC3 mutant in NB
Differential expression data set of S. coelicolor M145 and a abrC3 mutant cultured in NB. RNA samples were collected at 36, 48 and 60 hours following cDNA synthesis and labeling. The hybridization was conducted using DNA 104K microarrays (Surrey) using gDNA from S. coelicolor M145 as reference.
ORGANISM(S): Streptomyces coelicolor A3(2) 
2014-01-14 | E-MTAB-1453 | biostudies-arrayexpress
TranscriptomicsS.coelicolor_Rok7B7vsM145_MM+mannitol_time_course_expression
For transcript analysis, total RNA was isolated as described (Noens et al, 2007) from surface-grown mycelia of S. coelicolor M145 and its rok7B7 null mutant, cultivated on MM agar plates with mannitol (1% w/v) as the sole carbon source (plates were covered with cellophane discs). Two independent bio...
ORGANISM(S): Streptomyces coelicolor A3(2) 
2014-01-14 | E-MTAB-1465 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of S. coelicolor grown in liquid culture and heat shock stimulated
Global gene expression patterns of two biological replicate cultures of S.coelicolor MT1110 grown on YEME plus 10% sucrose up to early stationary phase ( the culture conditions were the same used for obtaining ChIP-chip data with anti HspR antibodies). RNA was harvested from heat-shocked cultures ...
ORGANISM(S): Streptomyces coelicolor 
2009-02-07 | E-MAXD-49 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Streptomyces coelicolor HspR binding sites
Global ChIP-chip analysis of HspR repressor binding sites in Streptomyces coelicolor MT1110 cultures grown in YEME plus 10% sucrose at 30?C up to early stationary phase. Chromatin extracted from formaldehyde treated cultures was incubated with anti HspR polyclonal antibodies and the DNA purified f...
ORGANISM(S): Streptomyces coelicolor 
2009-02-07 | E-MAXD-48 | biostudies-arrayexpress
TranscriptomicsGlobal distribution of RNA polymerase in Streptomyces coelicolor
Genome wide ChIP-chip analysis of RNA Polymerase binding sites in Streptomyces coelicolor MT1110 cultures grown in SMM + 0.5% casaminoacids at 30C up to mid exponential and stationary phase. Chromatin extracted from rifampicin and formaldehyde treated cultures was incubated with anti ? subunit of co...
ORGANISM(S): Streptomyces coelicolor 
2020-08-01 | E-MAXD-60 | biostudies-arrayexpress
TranscriptomicsTranscriptome data for Streptomyces coelicolor at 2 growth stage
Transcriptome analysis of Streptomyces coelicolor MT1110 cultures grown in SMM + 0.5% casaminoacids at 30?C up to mid exponential and stationary phase. Total RNA was extracted from both stages of growth, labelled with Cy3-dCTP and mixed with genomic DNA from S. coelicolor M145 labelled with Cy5-dCT...
ORGANISM(S): Streptomyces coelicolor 
2020-08-01 | E-MAXD-61 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of N,C and P limitations in S. coelicolor
A genome-wide transcriptomic analysis of the response of the model streptomycete Streptomyces coelicolor A3(2) M145 to fermentor culture in Modified Evans Media limited, respectively, for nitrogen, phosphate and carbon
ORGANISM(S): Streptomyces coelicolor A3(2) 
2011-05-01 | E-MAXD-59 | biostudies-arrayexpress
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