During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. Some of these effectors are ADP-ribosyltransferases that ADP-ribosylate host proteins to subvert plant innate immunity. The aim of this experiment was to identify ADP-ribos...
ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2. Enriched proteins were analyzed by LC-MS/MS. Non-tran...
ADP-ribosylated proteins were enriched using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w) from transgenic A. thaliana plants expressing the Pseudomonas syringae type III effectors AvrRpm1 or HopF2. A non-functional Af1521G42E mutant of the engineered ...
