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ProteomicsSupplementary Dataset S5. AvrRpm1 ADP-ribosylates MAMI when expressed in N. benthamiana.
During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to identify A...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD068485 | Pride
Lc-msms Arabidopsis thaliana Affinity purification Plant Pseudomonas syringae Adp-ribosylation Avrrpm1 Plant pathogen Type 3 effector 
ProteomicsSupplementary Dataset S6. AvrRpm1 ADP-ribosylates PLDGAMMA3 when expressed in N. benthamiana.
During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to identify A...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD068675 | Pride
Lc-msms Arabidopsis thaliana Affinity purification Plant Pseudomonas syringae Adp-ribosylation Avrrpm1 Plant pathogen Type 3 effector 
UnknownAvrRpm1 missense mutations weakly activate RPS2-mediated immune response in Arabidopsis thaliana.
Not available
S-EPMC3412798 | biostudies-literature
ProteomicsSupplementary Datasets S2 and S3. ADP-ribosylated peptides identified in three Af1521 Macro pulldown experiments from AvrRpm1-HA samples by the standard method (S2) and the 'triggering' method (S3).
During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to compare tw...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD068665 | Pride
Lc-msms Arabidopsis thaliana Affinity purification Plant Pseudomonas syringae Adp-ribosylation Avrrpm1 Plant pathogen Type 3 effector 
UnknownUntargeted Proteomics Identifies Plant Substrates of the Bacterial-Derived ADP-Ribosyltransferase AvrRpm1.
Not available
S-EPMC12620056 | biostudies-literature
UnknownSpecific resistances against Pseudomonas syringae effectors AvrB and AvrRpm1 have evolved differently in common bean (Phaseolus vulgaris), soybean (Glycine max), and Arabidopsis thaliana.
Not available
S-EPMC2922445 | biostudies-literature
ProteomicsPseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI)
Nicotiana benthamiana tissue expressing either AvrRpm1-3xFlag, StrepII-3xHA-MAMI, or co-expressing AvrRpm1-3xFlag and StrepII-3xHA-MAMI were subjected to enrichment of ADP-ribosylated proteins using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w). A non-...
ORGANISM(S): Nicotiana benthamiana 
2023-09-28 | PXD045549 | Pride
Arabidopsis thaliana Type iii effector Pseudomonas syringae Plant pathogen Adp-ribosylation Nicotiana bethamiana 
ProteomicsSupplementary Dataset S1. Proteins identified by untargeted proteomics in four Af1521 Macro pulldown experiments from Dex-treated Col-0, AvrRpm1-HA, and HopF2-HA plants.
During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. Some of these effectors are ADP-ribosyltransferases that ADP-ribosylate host proteins to subvert plant innate immunity. The aim of this experiment was to identify ADP-ribos...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD068442 | Pride
Hopf2 Lc-msms Arabidopsis thaliana Affinity purification Plant Pseudomonas syringae Adp-ribosylation Avrrpm1 Plant pathogen Type 3 effector 
ProteomicsSupplementary Dataset S4. Comparison of proteins identified in three experiments using the functional (Af1521) versus non-functional (G42E) Macro domains following ADPr-enrichment of proteins from Dex-treated AvrRpm1-HA plants.
During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to compare af...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD068551 | Pride
Lc-msms Arabidopsis thaliana Affinity purification Plant Pseudomonas syringae Adp-ribosylation Avrrpm1 Plant pathogen Type 3 effector 
ProteomicsPseudomonas type III effector AvrRpm1 ADP-ribosylates Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3)
Nicotiana benthamiana tissue expressing either PLDGAMMA3-3xHA-StrepII or co-expressing AvrRpm1-3xFlag and PLDGAMMA3-3xHA-StrepII were subjected to enrichment of ADP-ribosylated proteins using the engineered Af1521 Macro domain (Nowak et al., https://doi.org/10.1038/s41467-020-18981-w). A non-functio...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Nicotiana benthamiana 
2024-10-22 | PXD056607 | Pride
Arabidopsis thaliana Type iii effector Pseudomonas syringae Nicotiana benthamiana Plant pathogen Adp-ribosylation 
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