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Cells were lysed with TFA, and precipitated with cold acetone. The precipitates were transferred to E3filters and digestion was performed overnight with trypsin. The peptides were desalted with StageTips, and were analyzed on Eclipse Orbitrap with DIA mode. For MS1, the resolution was set to 120,00...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-02-27 | MSV000094179 | MassIVE
The goal of this study was to identify yeast mutants with arsenite hypersensitivity. Keywords: dose response Matched pools of yeast knockout (YKO) mutants were grown in paired growth media lacking or containing arsenite at various concentrations. The relative abundances of the YKO mutants were profi...
ORGANISM(S): Saccharomyces cerevisiae 
Here we used mass spectrometry-based proteomics technology to explore SEPs with potential cellular stress function in Saccharomyces cerevisiae. Microproteins with unique peptides were identified under six culture conditions: normal, oxidation, starvation, UV radiation, heat shock, and heat shock wit...
ORGANISM(S): Saccharomyces Cerevisiae 
2022-06-07 | PXD028623 |
This project investigates the molecular mechanisms by which blue light enhances conidiation in the medicinal fungus Shiraia bambusicola. We found that blue light not only advanced conidia production by 6 days but also increased the yield by over 7-fold. Through a comprehensive proteomic analysis, we...
ORGANISM(S): Shiraia Bambusicola 
2026-08-12 | PXD070936 |
293T cells were cultured in SILAC medium. Heavy group was labeled with K6&R10. Light group was labeled with K0&R0. In light group, cells were treated with MLN4924. In heavy group, cells were treated with DMSO. Then cells were lysed, mixed and tryptic digested. Peptide mixture was subjected to phosph...
ORGANISM(S): Homo Sapiens 
2021-06-29 | PXD027020 |
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