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MetabolomicsUHPLC-MS/MS Untargeted Metabolomics Profiling of Cecal Content in Three Chicken Breeds

This study applied an untargeted metabolomics approach to analyze cecal content samples from three chicken breeds (Wenchang chicken, White-ear Yellow chicken, and Recessive White chicken) using UHPLC-MS/MS (Vanquish UHPLC coupled with Q Exactive HFX Orbitrap). A total of 90 samples (30 per breed)...

2026-10-08 | MTBLS15811 | MetaboLights
ProteomicsIdentification of AF9-associated proteins by IP and mass spectrometry
To assess whether these proteins directly interact with each other in cells, we performed large-scale co-immunoprecipitation (co-IP) experiments using nuclear extract of HEL cells and the AF9 antibody against the N-terminus of AF9, and analyzed immunoprecipitated proteins by mass spectrometry. We id...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD042008 | Pride
Human Lc-ms/ms Immunoprecipitation Af9 Hel cells 
ProteomicsOligomerization switch in regulation of global transcription and DNA damage repair
In this study, using human AF9 as representative of Super Elongation Complex (SEC) components, we have studied detailed mechanisms of functional cross-talk between global transcriptional repression and efficient DNA damage repair during genotoxic stress.As a result of which we have identified intera...
ORGANISM(S): Homo sapiens (Human) 
2024-05-18 | PXD049385 | Pride
Human af9 ms/ms 
TranscriptomicsExpression changes after loss of Dot1l in murine MLL-AF9 leukemia cells
MLL-fusions may induce leukemogenic gene expression programs by recruiting the histone H3K79 methyltransferase to MLL-target promoters. We evaluated gene expression changes after cre-mediated loss of Dot1l in leukemia cells obtained from mice injected with MLL-9 transformed lineage negative bone mar...
ORGANISM(S): Mus musculus 
2011-09-12 | E-GEOD-25911 | biostudies-arrayexpress
MetabolomicsOGFOD1 enables AML chemo- and nutrient stress resistance by regulating protein synthesis

Acute Myeloid Leukemia (AML) commonly relapses after initial chemotherapy response. We assessed metabolic adaptations in chemoresistant cells in vivo before overt relapse, identifying altered branched-chain amino acid (BCAA) levels in patient-derived xenografts (PDX) and immunophenotypically iden...

2025-07-19 | MTBLS10255 | MetaboLights
TranscriptomicsConditional modeling of MLL-AF9 induced leukemia revealed a stem cell derived aggressive disease [RNA-Seq]
To address the impact of cellular origin on AML, we generated an inducible transgenic mouse model for MLL-AF9 driven leukemia. MLL-AF9 expression in long-term hematopoietic stem cells (LT-HSCs) in vitro resulted in unprecedented clonogenic growth and expression of genes involved in migration and inv...
ORGANISM(S): Mus musculus 
2016-06-27 | E-GEOD-65383 | biostudies-arrayexpress
TranscriptomicsAssesment of gene expression upon AF10 deletion in MLL-AF9 secondary leukemia
Aberrant Hox gene activation is a recurrent feature in several different types of human leukemia, including leukemias with rearrangements of the mixed lineage leukemia (MLL) gene. In this study, we demonstrate that Hox gene expression is controlled by higher degree H3K79 methylation in acute myeloid...
ORGANISM(S): Mus musculus 
2014-09-08 | E-GEOD-54499 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by high throughput sequencing of 7 tissues of maize inbred line Zheng 58
RNA-sequencing of 5 tissues of maize inbred line Zheng 58: mature silk (MS), mature pollen (MP), mature ovary (MO), and 6-days old seedling (SL), immature silk (IMS)
ORGANISM(S): Zea mays 
2013-01-26 | E-MTAB-964 | biostudies-arrayexpress
ProteomicsAffinity purification – mass spectrometry of AFF4 WT or S829/S831/3/5/8A mutant
The S831 phosphosite on AFF4 lies within a disordered region adjacent to the ENL/AF9 binding site. We therefore hypothesized that phosphorylation may regulate protein:protein interactions within the Super Elongation Complex. To investigate this, HEK293T cells expressing RFP negative control, FLAG-ta...
ORGANISM(S): Homo sapiens (Human) 
2025-12-16 | PXD054250 | Pride
Affinity purification Aff4 Interactome 
Proteomics3x-HA Tagged PAF1 IP-Ms in MOLM-13 CAS9 cells
Transcriptional regulatory proteins are frequent drivers of oncogenesis and common targets for drug discovery. The transcriptional co-activator, ENL, which binds to chromatin through an acetyllysine reader YEATS domain, is preferentially required for the survival and pathogenesis of acute leukemia. ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-21 | PXD066867 | Pride
Human Acute meyloid leukemia Transcriptional regulation 
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