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ProteomicsComparison of Transcript and Protein Abundance in Human Donor Retinas Support Extending Post-Mortem Interval from 12- to 18-hours to Expand the Donor Pool for Biomedical Research
Extending the post-mortem interval (PMI) from 12- to 18-hours was done to see if transcript and protein abundance in the human retina changed. Donor eyes were recovered from post-mortem donors (n=7 pairs) and stored at 2–8°C. Only donor eyes with ocular cooling within 8 hours of death were examined....
ORGANISM(S): Homo sapiens (Human) 
2026-04-07 | PXD068030 | Pride
Tmtpro Mass spectrometry Retina Orbitrap tribrid sps-ms3 acquisition Donor collection time Isobaric labeling quantitative proteomics 
ProteomicsCo-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma
Uveal melanoma (UM) is the most common intraocular tumor in adults, and its pathogenesis is only partly understood. UM can develop de novo from normal choroidal melanocytes (NCMs) or from pre-existing nevi that stem from NCMs and are thought to harbour UM-initiating mutations, most commonly in GNAQ ...
ORGANISM(S): Homo sapiens (Human) 
2024-11-25 | PXD047179 | Pride
Uveal melanoma Proteomics Primary cell culture Human donor eyes 
TranscriptomicsTranscription profiling of human donor-matched retinal and choroidal vascular endothelial cells
Consistent with clinical observations that posterior uveitis frequently involves the retinal vasculature and recent recognition of vascular heterogeneity, we hypothesized that retinal vascular endothelium was a cell population of unique molecular phenotype. Donor-matched cultures of primary retinal ...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7850 | biostudies-arrayexpress
TranscriptomicsGene expression of the human trabecular meshwork (TM) of the eye
We investigated the gene expression of the human TM. We isolated TM cells from healthy human donor eyes. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choro...
ORGANISM(S): Homo sapiens 
2013-09-12 | E-GEOD-50784 | biostudies-arrayexpress
TranscriptomicsDexamethasone induced gene expression changes in the human trabecular meshwork
One of the undesirable side effects of glucocorticoid use is the potential development of elevated intraocular pressure and glaucoma. The precise mechanisms through which steroid use induces ocular hypertension are unclear. The purpose of this study was to identify gene expression changes induced by...
ORGANISM(S): Homo sapiens 
2012-10-01 | E-GEOD-37474 | biostudies-arrayexpress
MetabolomicsQuantitative metabolomic analysis of the human cornea and aqueous humor
INTRODUCTION: Cornea is the outermost part of the eye supplied mostly by aqueous humor (AH). Therefore, the comparison of the metabolomic compositions of AH and cornea may help to determine which compounds are produced inside the cornea, and which penetrate into cornea from AH for intra-corneal cons...
2018-06-20 | MTBLS444 | MetaboLights
TranscriptomicsEffects of Aging and Anatomic Location on Gene Expression in Human Retina
Objective: To determine the effects of age and topographic location on gene expression in human neural retina. Methods: Macular and peripheral neural retina RNA were isolated from human donor eyes for DNA microarray and quantitative RTPCR analyses. Results: Total RNA from human donor retina preserve...
ORGANISM(S): Homo sapiens 
2015-05-27 | E-GEOD-32614 | biostudies-arrayexpress
GenomicsSingle-nuclei RNAseq (NucSeq) of human eyes
Single-nuclei RNAseq (NucSeq) of human eyes
PRJNA557496 | ENA
ProteomicsProteomics of Primary Uveal Melanoma:Insights to Metastasis and Protein Biomarkers
Uveal melanoma metastases are lethal and remain incurable. Quantitative proteomic analysis of 53 metastasizing and 47 non-metastasizing primary uveal melanoma (pUM) was pursued for insights into UM metastasis and protein biomarkers. The metastatic status of the pUM specimens was defined based on cli...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-05-15 | MSV000087437 | MassIVE
TranscriptomicsMicroRNA Expression in Human RPE Cells in Culture: Control vs Treated with IFN-? + TNF-? + IL-1?
Human retinal pigment epithelial (HRPE) cells in culture respond to inflammatory cytokines (IFN-? + TNF-? + IL-1? ) by increasing the expression of many cytokines and chemokines. The goal of this study was to delineate the role of miRNA in this process. We employed microarray analysis to study the e...
ORGANISM(S): Homo sapiens 
2010-11-11 | E-GEOD-23979 | biostudies-arrayexpress
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