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Arabidopsis thaliana Legumain (aka VPE) beta / gamma were characterized for their cleavage specificity and putative substrates were identified by the N-termini enrichment method HUNTER.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-07-29 | PXD019276 | Pride
Optimization hydrophobic tagging reaction time (30 min to 2h) and evaluation of the HUNTER protocol performance with different amounts (25 ug to 400 ug) of purified rat brain proteome as starting material.
ORGANISM(S): Rattus norvegicus (Rat) 
2019-09-03 | PXD012844 | Pride
The mammalian lysosomal protease legumain is often dysregulated in pathophysiological conditions including inflammation, neurodegeneration, and cancer, yet its proteolytic targets are poorly defined. To profile protease substrates degradomics techniques typically employ enrichment strategies to sele...
ORGANISM(S): Mus musculus (Mouse) 
2025-09-29 | PXD060854 | Pride
We utilized ChIP Seq to examine the the genomic localization of the increase in H3K9 trimethylation we had previously observed in the hippocampus as a consequence of acute restraint stress in rats (Hunter et. al PNAS 2009). Rats were restrained for 30 minutes, allowed to recover for 1 hour and then ...
ORGANISM(S): Rattus norvegicus 
Enterocyte–ILC1 crosstalk drives innate IFN-g-mediated control of Cryptosporidium
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