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ProteomicsDetergent Screening with Hybrid Detergents Increases Observable Number of Protein Identities in Bottom-Up Proteomics
Detergents are key reagents in bottom-up proteomics that create an apparent, yet underappreciated bias on observable proteomes. Maximizing the chemical diversity in parallelized detergent screens is supposed to maximize observable proteomes by combining proteomics data sets of different detergents. ...
ORGANISM(S): Escherichia coli 
2025-08-25 | PXD060875 | Pride
Proteomics E. coli Bottum-up Hybrid detergent Sds 
UnknownDetergent Screening With Hybrid Detergents Increases Observable Number of Protein Identities in Bottom-Up Proteomics.
Not available
S-EPMC12329390 | biostudies-literature
UnknownDetergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs.
Not available
S-EPMC9097535 | biostudies-literature
GenomicsRapid Genome-Scale Mapping of Chromatin Accessibility in Tissue
Here we introduce a novel technique that specifically identifies Tissue Accessible Chromatin (TACh). The TACh method uses pulverized frozen tissue as starting material and employs one of the two robust endonucleases, Benzonase or Cyansase, which are fully active under a range of stringent conditions...
ORGANISM(S): Mus musculus 
2012-08-10 | E-GEOD-39982 | biostudies-arrayexpress
ProteomicsNative Mass Spectrometry of Soluble Proteins with Sodium Chloride enabled by a Dendritic Detergent
Salts are pivotal for ligand binding but hamper the analysis of proteins through native mass spectrometry due to adduct formation and consequent signal suppression. Here, we show how to facilitate native mass spectrometry of soluble proteins and protein–ligand complexes in the presence of sodium chl...
ORGANISM(S): Bos taurus (Bovine) Oryctolagus cuniculus (Rabbit) Pseudomonas aeruginosa PAO1 Saccharomyces cerevisiae (Baker's yeast) Equus caballus (Horse) 
2026-09-14 | PXD074233 | Pride
UnknownAn automated data processing and analysis pipeline for transmembrane proteins in detergent solutions.
Not available
S-EPMC7228933 | biostudies-literature
ProteomicsTargeted quantification of detergent-insoluble RNA-binding proteins in Alzheimer’s disease reveals disease-specific coaggregation signatures
Core spliceosome and related RNA-binding proteins aggregate in Alzheimer’s disease (AD) brain even in early asymptomatic stages (AsymAD) of disease. To assess the specificity of RNA-binding protein aggregation in AD, we developed a targeted mass spectrometry approach to quantify broad classes of RNA...
ORGANISM(S): Homo sapiens (Human) 
2021-03-17 | PXD022144 | Pride
Parkinson’s disease Alzheimer’s disease Insoluble proteins Rna-binding proteins Parallel reaction monitoring (prm) mass spectrometry Human brains 
ProteomicsTMT analysis of detergent-soluble and insoluble fractions from HEK293T cells in normal conditions and upon heat shock
HEK293T cells were cultured according to standard protocols and heat shocked at 42oC for 6 hours. Detergent-soluble and insoluble fractions from HEK293T cells were obtained following the procedures described before. Specifically, HEK293T cells were lysed in NP40 lysis buffer (Invitrogen #FNN0021) wi...
ORGANISM(S): Homo sapiens (Human) 
2025-08-01 | PXD046967 | Pride
Proteome solubility  thermal stress  heat shock  hek293 cells 
UnknownAccumulation of protein aggregates induces autolytic programmed cell death in hybrid tobacco cells expressing hybrid lethality.
Not available
S-EPMC6629611 | biostudies-literature
UnknownThe effect of PRMT1-mediated arginine methylation on the subcellular localization, stress granules, and detergent-insoluble aggregates of FUS/TLS.
Not available
S-EPMC3496700 | biostudies-literature
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