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ProteomicsA Tribrid Hyperplexing approach for Large-scale Quantitative Proteomic Analysis
Isobaric labeling has emerged as an indispensable quantitative proteomic approach for its unprecedented multiplexing capacity in a single analysis. Currently, different hyperplexing approaches have been developed to meet the need for increasing sample numbers in large-scale cohort analysis. In this ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2023-03-17 | PXD037498 | Pride
Tmtpro Ibt Tmt Hyperplexing Quantitative proteomics 
UnknownDataset generated using hyperplexing and click chemistry to monitor temporal dynamics of newly synthesized macrophage secretome post infection by mycobacterial strains.
Not available
S-EPMC5030312 | biostudies-literature
UnknownIncreasing the Throughput and Reproducibility of Activity-Based Proteome Profiling Studies with Hyperplexing and Intelligent Data Acquisition.
Not available
S-EPMC11301772 | biostudies-literature
ProteomicsMycobacterial strain-specific temporal modulations of newly synthesized macrophage secretome
THP-1 macrophages were infected with four strains of Mycobacterium tuberculosis to study the temporal dynamics of newly synthesized proteins in the secretome. Temporal snapshots of secretome reflect the macrophage response to pathogenicity which in combination with intracellular events, completes th...
ORGANISM(S): Homo sapiens (Human) 
2016-08-10 | PXD004281 | Pride
Boncat Mtb infection Hyperplexing Newly synthesized Silac Temporal Itraq Secretome 
ProteomicsTime-resolved analysis of proteome dynamics by TMT-SILAC hyperplexing
In this project, a more efficient methodology for obtaining multi-timepoint kinetic data for proteome-wide analyses of protein turnover is developed and used to globally measure the kinetics of protein turnover in primary human fibroblasts (HCA2-hTert). This approach takes advantage of the multiplex...
ORGANISM(S): Homo sapiens (Human) 
2016-12-14 | PXD004725 | Pride
Tmt-silac Protein degrdation Tmt Proteome dynamics Silac Human primary fibroblasts Protein synthesis Hyperplexing 
ProteomicsUltraPlex: Expanding isobaric hyperplexing via orthogonal protease cleavage
Sample preparation was conducted using an adaptation of our SimPLIT workflow10. Four cell pellets corresponding to four different colorectal cancer cell lines were lysed in a buffer containing 1% sodium deoxycholate (SDC, Merck, Cat. No. 30970), 100 mM triethylammonium bicarbonate (TEAB, Merck, Cat....
ORGANISM(S): Homo sapiens (Human) 
2026-01-30 | PXD070352 | Pride
ProteomicsTAG-TMTpro, a hyperplexing quantitative approach for high-throughput proteomic studies
Isobaric labeling is the most widely used multiplexing quantitative approach in proteomic studies, enabling the comparison of up to 18 samples in a single MS analysis. Expanding the multiplexing capacity is of great necessity for high-throughput proteomic studies. Herein, we establish a novel TAG-TM...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2022-10-14 | PXD033711 | Pride
Proteomics Multiplexing quantitative approach Tmtpro labeling 
ProteomicsIncreasing the Throughput and Reproducibility of Activity-Based Proteome Profiling Studies with Hyperplexing and Intelligent Data Acquisition
Intelligent data acquisition (IDA) strategies, such as real-time database search (RTS), have improved the depth of proteome coverage for experiments that utilize isobaric labels and gas phase purification techniques (i.e., SPS-MS3). While most applications of IDA have been focused on the analysis o...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2023-06-06 | MSV000092113 | MassIVE
UnknownHyperQuant-A Computational Pipeline for Higher Order Multiplexed Quantitative Proteomics.
Not available
S-EPMC7240821 | biostudies-literature
UnknownHyperplexing: a method for higher-order multiplexed quantitative proteomics provides a map of the dynamic response to rapamycin in yeast.
Not available
S-EPMC5292868 | biostudies-literature
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