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GenomicsFlexible chromatin loops in the VH gene region of the Igh locus facilitate the generation of a diverse antibody repertoire
The immunoglobulin heavy-chain (Igh) locus undergoes large-scale contraction in pro-B cells, which facilitates VH-DJH recombination by juxtaposing distal VH genes next to the DJH- rearranged gene segment in the proximal Igh domain. By high-resolution mapping of long-range interactions, we now demons...
ORGANISM(S): Mus musculus 
2013-09-30 | E-GEOD-43008 | biostudies-arrayexpress
GenomicsPTIP promotes chromatin changes critical for immunoglobulin class switch recombination
Programmed genetic rearrangements in lymphocytes require transcription at antigen receptor genes to promote accessibility for initiating double-strand break (DSB) formation critical for DNA recombination and repair. Here we show that activated B cells deficient in the PTIP component of the MLL3 (mix...
ORGANISM(S): Mus musculus 
2010-08-25 | E-GEOD-20852 | biostudies-arrayexpress
TranscriptomicsAllele specific analysis of the immunoglobulin heavy chain locus by simultaneous analysis of the productive and the non-productive allele through paired-end 4C sequencing analysis in mature B cells
In developing B cells the immunoglobulin heavy chain (IgH) locus is thought to move from repressive to permissive chromatin compartments to facilitate its scheduled rearrangement. In mature B cells, maintenance of allelic exclusion has been proposed to involve recruitment of the non-productive IgH a...
ORGANISM(S): Mus musculus 
2013-06-01 | E-GEOD-47128 | biostudies-arrayexpress
TranscriptomicsAllele specific analysis of the immunoglobulin heavy chain locus
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2013-06-01 | E-GEOD-47129 | biostudies-arrayexpress
GenomicsHistone Reader BRWD1 Targets and Restricts Recombination to the Igk Locus
B lymphopoiesis requires that immunoglobulin genes be accessible to RAG1-RAG2 recombinase. However, the RAG proteins bind widely to open chromatin, which suggests that additional mechanisms must restrict RAG-mediated DNA cleavage. Here we show that developmental downregulation of interleukin 7 (IL-7...
ORGANISM(S): Mus musculus 
2015-08-24 | E-GEOD-63302 | biostudies-arrayexpress
TranscriptomicsPre-BCR Signaling induce IgK Locus Accessibility by functional redistribution of Enhancer-mediated chromatin Interactions
During B cell development the precursor B cell receptor (pre-BCR) checkpoint is thought to increase immunoglobulin k light chain (Igk) locus accessibility to the V(D)J recombinase. Accordingly, pre-B cells lacking the pre-BCR signaling molecules Btk or Slp65 showed reduced germline Vk transcription....
ORGANISM(S): Mus musculus 
2014-03-21 | E-GEOD-53896 | biostudies-arrayexpress
TranscriptomicsIGH analysis in pro-B cells with H3K4me3, H3K4me2 and H3K9ac
STAT5 and IL-7 signaling are thought to control B-lymphopoiesis by regulating key transcription factor genes and activating VH gene segments at the Igh locus. Using conditional mutagenesis, we demonstrate that transgenic Bcl2 expression rescued the development of Stat5-deleted pro-B cells by compens...
ORGANISM(S): Mus musculus 
2010-07-28 | E-GEOD-18278 | biostudies-arrayexpress
TranscriptomicsNon-coding transcription within the Igh distal VH region at PAIR elements affects the 3D structure of the Igh locus in pro-B cells
Non-coding sense and antisense germline transcription within the immunoglobulin heavy chain locus precedes V(D)J recombination and has been proposed to be associated with Igh locus accessibility, although its precise role remains elusive. However, no global analysis of germline transcription through...
ORGANISM(S): Mus musculus 
2012-10-18 | E-GEOD-40984 | biostudies-arrayexpress
TranscriptomicsExpression data for resting and activated B and T cells
The IgH locus encodes for part of the antibody exposed by B cells and is important for the immune system. In B cells, one allele produces protein, the other must remain silenced. It was proposed that both alleles reside in different nuclear compartments and that this is important to maintain mono-al...
ORGANISM(S): Mus musculus 
2013-06-01 | E-GEOD-46865 | biostudies-arrayexpress
ProteomicsInfluence of immunoglobulin genotypes on the elicitation of SARS CoV-2 neutralizing antibodies
The human immunoglobulin heavy chain (IGH) locus is exceptionally polymorphic with high allelic diversity of variable (V) genes and structural variation affecting large regions of the locus. Thus, our germline IGHV gene and allele content is highly personal, which may influence how we respond to inf...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD031945 | Pride
Neutralizing antibody Sars-cov-2 Immunoglobulin germline allele Single nucleotide polymorphism Copy number variation Hdx-ms 
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