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ProteomicsUpdated in-gel digestion for protein analysis by mass spectrometry
The in-gel digestion of proteins for analysis by liquid chromatograph mass spectrometry has been used since the early 1990s. Although several improvements have contributed to increasing the quality of the data obtained, many recent publications still use sub-optimal approaches. We present an updated...
ORGANISM(S): Homo sapiens (Human) 
2018-10-02 | PXD009600 | Pride
Proteomics Mass spectrometry In-gel digestion Trypsin 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009485 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
ProteomicsBAC-DROP: Rapid digestion of proteome fractionated via dissolvable polyacrylamide gel electrophoresis and its application to bottom-up proteomics workflow
The GeLC-MS workflow, which combines low-cost, easy-to-use SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with liquid chromatography-mass spectrometry (LC-MS), is very popular in current bottom-up proteomics. However, GeLC-MS requires that PAGE-separated proteins undergo overnight enzymatic diges...
ORGANISM(S): Homo Sapiens Hepatitis B Virus Bos Taurus 
2021-01-24 | PXD021489 | panorama
ProteomicsTube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples
Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-10-18 | PXD003841 | Pride
Label-free quantitative proteomics Liquid digestion Sample preparation Tube-gel Stacking gel 
ProteomicsBAC-DROP: Rapid digestion of proteome fractionated via dissolvable polyacrylamide gel electrophoresis and its application to bottom-up proteomics workflow
The GeLC-MS workflow, which combines low-cost, easy-to-use SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with liquid chromatography-mass spectrometry (LC-MS), is very popular in current bottom-up proteomics. However, GeLC-MS requires that PAGE-separated proteins undergo overnight enzymatic diges...
ORGANISM(S): Homo Sapiens (human) 
2021-09-17 | PXD021523 | JPOST Repository
UnknownHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Not available
S-EPMC5988721 | biostudies-literature
TranscriptomicsBenign prostate epithelial cell gene expression after four weeks of treatment with daily transdermal DHT or placebo gel
Significant increases in serum DHT do not increase intraprostatic levels of DHT, PSA, prostate volume, or prostate epithelial cell androgen-regulated gene expression in healthy men. Changes in serum androgen concentrations are not necessarily mimicked within the prostate. Custom Agilent 44K whole hu...
ORGANISM(S): Homo sapiens 
2011-01-19 | E-GEOD-20317 | biostudies-arrayexpress
UnknownCentrifugal methods and devices for rapid in-gel digestion of proteins.
Not available
S-EPMC2907145 | biostudies-literature
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