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ProteomicsFilamin C domain 18-21 in vitro kinase assay
In vitro kinase assay with human filamin C domain 18-21 recombinantly expressed in E.coli, purified by Ni2+-NTA beads and incubated with PKC alpha, Akt or a combination of both to identify kinase specific phosphorylation sites.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-25 | PXD008893 | Pride
Filamin c Akt In vitro kinase assay Pkb Pkcalpha 
ProteomicsBead-based kinase phosphorylation profiling identifies SRC as a therapeutic target in glioblastoma
Here we describe a bead-based method capable of profiling tyrosine kinase phosphorylations in a multiplexed, high-throughput and low-cost manner. This approach allows for the discovery of tyrosine kinase-activating events, even when the DNA sequence is wild-type. In an effort to pilot the establishm...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-13808 | biostudies-arrayexpress
ProteomicsIdentification of phosphorylation sites of purified ORP8 from in vitro AMPK kinase assay
We investigated the potential role of AMPK in the phosphorylation of ORP8.In vitro kinase assay using purified ORP8 and kinase-active AMPK complex confirmed that ORP8 is the direct substrate of AMPK. Phosphorylated ORP8 from in vitro kinase assay was analyzed by HPLC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD036201 | Pride
Ampk Phosphorylation Orp8 
ProteomicsPhosphorylation and protein interactions in plant gravity sensing
We studied the phosphorylation of Arabidopsis LAZY4 proteins during gravistimulation, and searched for its interaction proteins using Immunoprecipitation-Mass Spectrometry (IP-MS). For the identified kinases MPK3 and MKK5, we carried out in vitro kinase assays using recombinant LAZY4 as the substrat...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-09-20 | PXD045213 | Pride
Lazy Gravitropism Mpk3 Phosphorylation Mkk5 
ProteomicsProteomic profiling of human monocytes stimulated in vitro with N. meningitidis
Meningococcal sepsis is an overwhelming form of the sepsis syndrome which may cause mortality within 12-24 hours in previously healthy children and adults, where the causative infectious agent is N. meningitidis, an obligate human pathogen. The genomic changes induced by N. meningitidis are modulate...
ORGANISM(S): Homo sapiens 
2017-12-15 | E-MTAB-4795 | biostudies-arrayexpress
TranscriptomicsExpression data from P14 TCR cytotoxic T cells overexpressing HDAC7 phosphorylation deficient mutant
The present study reports an unbiased analysis of the cytotoxic T cell serine-threonine phosphoproteome using high resolution mass spectrometry. Approximately 2,000 phosphorylations were identified in CTLs of which approximately 450 were controlled by TCR signaling. A significantly overrepresented g...
ORGANISM(S): Mus musculus 
2011-03-13 | E-GEOD-27092 | biostudies-arrayexpress
ProteomicsIdentification of the Kinase phosphorylation sites on AtGPA1 in presence of GTP
Phosphorylation of GPA1 has been shown to functionally important in vitro and in vivo. We previously identified18 LRR RLKs transphosphorylation of AtGPA1 via in vitro kinase assay using recombinant kinase domains and AtGPA1 (Bo et al., J Biol Chem 293(13): 4752-4766). Among them, we choose 12 kinase...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2019-06-12 | MSV000083964 | MassIVE
ProteomicsNaringenin targets PI3K p85alpha to suppress PI3K/AKT signaling pathway and ameliorate disordered MMP-9 secretion in cigarette smoke extract-induced alveolar macrophages in vitro
Naringenin has demonstrated potential therapeutic effects against cigarette smoke-induced lung injury; however, its underlying mechanisms of regulating matrix metalloproteinase-9 (MMP-9) in alveolar macrophages remain unclear. Methods: The regulatory mechanisms of naringenin in cigarette smoke extra...
ORGANISM(S): Mus Musculus 
2025-04-21 | PXD063137 |
ProteomicsIdentification of phosphorylation sites of transcription factor after reaction with protein kinase
In previous study, we reported that MdERF17 contributes to Chl degradation in apple fruit peels. Here, we demonstrated that MdERF17 and MdMPK4 interact with each other in vitro and in vivo and raised the possibility that MdMPK4 may contribute to fruit degreening via post-transcriptional regulation o...
ORGANISM(S): Malus domestica 
2022-05-20 | PXD030037 | Pride
Phosphorylation，in vitro kinase assay，lc-ms/ms 
ProteomicsIdentification of the Kinase phosphorylation sites on AtGPA1 in presence of GDP
Phosphorylation of GPA1 has been shown to functionally important in vitro and in vivo. We previously identified18 LRR RLKs transphosphorylation of AtGPA1 via in vitro kinase assay using recombinant kinase domains and AtGPA1 (Bo et al., 2018, J. Biol. Chem 293(13): 4752-4766). Among them, we here cho...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2019-06-12 | MSV000083963 | MassIVE
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