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ProteomicsIdentification of zein proteome by multi-enzyme digestion
Zeins are a class of prolamine proteins extracted from maize, and are extensively used in the food and pharmaceutical industries. Therefore, characterization of its components is essential for quality control and safety evaluation. However, zein proteins have low abundance of the trypsin targets lys...
ORGANISM(S): Zea Mays 
2019-07-25 | PXD014743 |
ProteomicsProteomics Using Proteases Alternative to Trypsin Benefits from Sequential Digestion with Trypsin
High specificity and ease of use make trypsin the most used enzyme in proteomics. Proteases with complementary cleavage specificity to trypsin have been applied to obtain additional data. However, use of proteases with broad specificity proved especially challenging. In this work, we analyzed the ch...
ORGANISM(S): Schizosaccharomyces pombe OY26 
2021-06-07 | PXD017321 | Pride
Alternative proteases Sequential digest 
ProteomicsMS characterization of spidroin-1
For MS characterization of major ampullate spidroin-1 from the three species of Nephila clavipes, edulis and madagascariensis, an experimental approach was developed combining 2-DE with multiple proteolytic in-gel digestion (trypsin, chymotrypsin, pepsin, proteinase K, subtilisin and proteinase 1...
ORGANISM(S): Nephila clavipes (Golden silk orbweaver) 
2022-02-22 | PXD000057 | Pride
Major ampullate silk Nephila sp. Post-translational modifications Phosphorylation Nanolc-esi-cid/etd-ms/ms Spidroin-1 
OtherGel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro.
Data from ProteomeXchange, PXD ID: PXD000311. Enzyme: trypsin, file: F017572.dat-ACA-SDS_T3.mzml. Published as part of Proteomics. 2014 Jul 10 . From the Abstract: {{i}} ... Proteins were extracted from pooled whole mouse brains, enriched membrane fractions were prepared using either two commercial...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32310002988 | GPMDB
Resource Reanalysis 
UnknownImproved protease digestion conditions for membrane protein detection.
Not available
S-EPMC4739822 | biostudies-literature
ProteomicsMALDI MS-MS analysis of Arachis hypogaea seed proteinase inhibitor
Plant Protease Inhibitors (PIs) plays an important role in defense and combat against insect pests and pathogens. In the present study, Bowman-Birk Inhibitor (BBI) was purified(���������8 kDa) from mature seeds of an interspecific advanced hybrid peanut variety (4368-1) using trypsin affinity and si...
ORGANISM(S): Arachis hypogaea (Peanut) 
2020-02-18 | PXD016933 | Pride
Maldi tof-tof Two-dimensional electrophoresis Arachis hypogaea Peanut bowman-birk inhibitor (pnbbi) 
ProteomicsAnalysis of mouse hippocampus membrane proteome by gel-based LC/MS
Mouse hippocampus membrane fractions were prepared using sucrose density gradient ultracentrifugation. Membrane proteins were solubilized using three different condition; 6-ACA/Triton X-100 or ProteoExtract Native Membrane Protein Extraction Kit (Calbiochem, Cat. No. 444810) or ProteoExtract Transme...
ORGANISM(S): Mus musculus (Mouse) 
2014-08-15 | PXD000311 | Pride
Mouse hippocampus Ltq orbitrap velos pro Membrane proteins Gel-based proteomics In-gel chymotrypsin/trypsin digestion 
ProteomicsLysine Methylation Promotes VEGFR-2 Activation and Angiogenesis
We have used a chimeric VEGFR-2 in which the extracellular domain of mouse VEGFR-2 was replaced with the extracellular domain of human CSF-1 receptor. VEGFR-2 was immunoprecipitated with anti-VEGFR-2 antibody from PAE cells ectopically expressing VEGFR-2. The immunoprecipitated proteins were eluted...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-11-22 | PXD000520 | Pride
In-gel digestion Lc-ms/ms 
ProteomicsMembrane Fraction Complexome of Nitrososphaera viennensis with BN-PAGE and Examination of the Archaeal Ammonia Monooxygenase Complex via SDS-Tricine-PAGE
Information on the files related to the respective experiments can be found in the metadata file titled “Metadata_for_Nviennensis_Experiments.xlsx”. The data found here encompasses three related mass spectrometry experiments: 1.) BN-PAGE: Biomass of Nitrososphaera viennensis grown in a bioreactor w...
ORGANISM(S): Nitrososphaera viennensis EN76 
2023-02-13 | PXD035349 | Pride
Ammonia oxidizing archaea Ammonia monooxygenase Copper membrane cound monooxygenase Nitrification Archaea Ammonia oxidation 
ProteomicsIdentification and Characterization of a Pepsin and Chymotrypsin-Resistant Peptide in the α Subunit of the 11S Globulin Legumin from Common Bean (Phaseolus vulgaris L.)
The 11S globulin legumin typically accounts for approximately 3% of total protein in common bean (Phaseolus vulgaris). It was previously reported that a legumin peptide of approximately 20 kDa is resistant to pepsin digestion. Sequence prediction suggested that the pepsin resistant peptide is locate...
ORGANISM(S): Phaseolus vulgaris (Kidney bean) (French bean) 
2024-08-09 | PXD046332 | Pride
Resistance to proteolytic digestion 11s globulin Legumin Pepsin Phaseolus vulgaris Chymotrypsin Common bean 
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