The integration of cell metabolism with signalling pathways, transcription factor networks and epigenetic mediators is critical in coordinating molecular and cellular events during embryogenesis. Induced pluripotent stem cells (IPSCs) are an established model for embryogenesis, germ layer specificat...
Cardiovascular disease is a leading cause of death worldwide. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold immense clinical potential and recent studies have enabled generation of virtually pure hPSC-CMs with high efficiency in chemically defined and xeno-free conditions. Despi...
Human induced pluripotent stem cells were differentiated into NSC for 7 days. Cells on days 0, 1, 2, 3, 5 and 7 of differentiation (2.0 × 10⁶) were washed twice with DPBS (−) and then mixed with 100 μL of dissolution buffer consisting of 12 mM sodium deoxycholate, 12 mM N-lauroylsarcosine, 100 mM Tr...
Reprogrammed somatic cells offer a valuable source of pluripotent cells that have the potential to differentiate into many cells types and provide a new tool for regenerative medicine. In the present study we differentiated induced pluripotent stem cells (iPS cells) into hepatic cells. We first show...
Rapid and deep profiling of human induced pluripotent stem cell proteome analysis by one-shot nano LC-MS/MS with meter-scale monolithic silica capillary columns.
This SuperSeries is composed of the following subset Series: GSE23402: Chromatin Structure and Gene Expression Programs of Human Embryonic and Induced Pluripotent Stem Cells (Affymetrix) GSE23455: Chromatin Structure and Gene Expression Programs of Human Embryonic and Induced Pluripotent Stem Cells ...
Single-cell RNA-seq (scRNA-seq) on nocodazole and DMSO treated cells before and after differentiation into endoderm. hPSC colonies were treated with DMSO or 100ng/ml nocodazole for 16 hours and induced to differentiate into definitive endoderm for three days. Single cells were subsequently collected...
Conventional embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) derived from primates resemble mouse epiblast stem cells, raising an intriguing question regarding whether the naïve pluripotent state resembling mouse embryonic stem cells (mESCs) exists in primates and how to captur...
Self-renewal of tendons is rare since the vascular formation inside is extremely poor, thus reconstructive surgery using autologous tendons has been often taken place in the case of severe injury. However, the rate of re-injury after surgery is relatively high, and collection of autologous tendons l...
