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ProteomicsAnti-FLAG co-immunoprecipitation of N- or C-terminally FLAG-HA-tagged FAM98A, FAM98B and FAM98C, from HEK Flp-In T-REx 293 cells.
Determination of the interactome of different human FLAG-HA-tagged FAM98 paralogs (FAM98A, FAM98B and FAM98C) by co-immunoprecipitation/MS. Two expression constructs were used per paralog: one with an N-terminal and one with a C-terminal FLAG-HA-tag. Expression constructs were stably integrated into...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD076703 | Pride
TranscriptomicsNPAS3-HEK293-circadian-Illumina
The fragments of FLNPAS3 and DeltaNPAS3 were transferred into a similar TET-inducible expression plasmid and transfected into HEK293 [T-REx-293] cells (Invitrogen). At the zero hour time-point, cells were shifted into a serum-rich medium plus tetracycline in order to induce circadian cycling and NPA...
ORGANISM(S): Homo sapiens 
2011-10-03 | E-TABM-1079 | biostudies-arrayexpress
TranscriptomicsExpression data of inducible stable cell lines overexpressing Ero1alpha WT and Ero1alpha C104A/C131A
The overall cellular response to oxidative stress generated by Ero1M-NM-1 in the lumen of the mammalian endoplasmic reticulum (ER) is poorly characterized. Here, we investigate the effects of overexpressing a hyperactive mutant (C104A/C131A) of Ero1M-NM-1. Using microarray analysis, we demonstrate t...
ORGANISM(S): Homo sapiens 
2012-09-07 | E-GEOD-40601 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of the effect of elevated TRIB3 expression on the transcriptional response to glucose deprivation
Gene expression profiling of HEK293-derived cell lines that are stably transfected with either a tetracycline-inducible TRIB3 expression construct (TRIB3-293 cells), tetracycline-inducible ATF4 expression construct (ATF4-293 cells) or the corresponding empty vector (Vector-293 cells). Samples from t...
ORGANISM(S): Homo sapiens 
2016-02-09 | E-MTAB-3318 | biostudies-arrayexpress
ProteomicsAIFM1 KO and AIFM1del mutant HEK cell proteomics
Mitochondrial proteases regulate the dynamic properties of the organelle morphology and ensure functional plasticity at the cellular level. The metalloprotease OMA1 mediates constitutive and stress-inducible processing of its substrates in mitochondria, but the number of functionally characterized s...
ORGANISM(S): Homo sapiens (Human) 
2026-01-31 | PXD063602 | Pride
Aifm1 Mitochondrion Hek 
TranscriptomicsiCLIP of TDP-43
A stable HEK293 FlpIn T-Rex cells expressing TDP-43 with an N-terminal eGFP-tag was generated that allowed inducible physiological expression of the protein (Ling et al. 2010). Duplicate iCLIP experiments were performed using an antibody targeting eGFP (Abcam ab290). Crosslinked RNA-protein complexe...
ORGANISM(S): Homo sapiens 
2017-05-01 | E-MTAB-4733 | biostudies-arrayexpress
Transcriptomicseffect of expression of a dominant negative HSF1 mutant
Expression of a dominant negative HSF1 mutant (HSF379) was induced in HEK293 cells and the effect of the transcriptome of non-stressed cells was monitored
ORGANISM(S): Homo sapiens 
2012-03-01 | E-MEXP-3561 | biostudies-arrayexpress
ProteomicsIdentification of ADAR2-associated complexes
Adenosine deaminase acting on RNA 2 (ADAR2) catalyzes adenosine-to-inosine RNA editing and contributes to multiple aspects of post-transcriptional regulation. Proximity-labeling (BioID) studies have previously defined the proximal protein environment of ADAR2. To complement these datasets and identi...
ORGANISM(S): Homo sapiens (Human) 
2026-04-30 | PXD071677 | Pride
Human Hek flp-in 293 t-rex™ cell Affinity purification Adar2 wt-ha-strepii 
UnknownApolipoprotein L1 (APOL1) cation current in HEK-293 cells and in human podocytes.
Not available
S-EPMC10321438 | biostudies-literature
TranscriptomicsAmplicons of a ND5 mRNA segment to detect m1A1374 levels in HEK pTRMT10C cells and control cells
This experiment was set up to assess the effect of TRMT10C overexpression on m1A methylation levels at position 1374 of the mitochondrial encoded ND5 mRNA. To investigate if TRMT10C is the writer enzyme for this m1A site, a cell system providing tetracycline-inducible TRMT10C expression was used (te...
ORGANISM(S): Homo sapiens 
2023-07-24 | E-MTAB-12038 | biostudies-arrayexpress
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