Inhibitory interneurons

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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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Department of Biology, University of Napoli Federico II, Napoli, Italy (1)
Department of Fundamental Neurosciences (DNF) University of Lausanne Rue du Bugnon 9 CH-1005 Lausanne Switzerland (1)
Head of the Mass Spectrometry and Proteomics facility (LSMP) at Institut Curie, France (1)
Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Germany (1)
Max-Planck Institute of Psychiatry (1)

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LFQ proteomics of mouse GABAergic interneurons during traumatic brain injury

Unilateral Traumatic Brain Injury (TBI) causes functional disturbances of the neuronal networks spreading even to the undamaged cortical hemisphere. The phenomenon, referred to as transhemispheric diaschisis, is suggested to be mediated by an imbalance of the strength of glutamatergic, excitatory vs...

ORGANISM(S): Mus musculus (Mouse)

2022-01-03 | PXD023551 | Pride

Nr4a1 regulates cell-specific transcriptional programs in inhibitory GABAergic interneurons

PRJNA1095557 | ENA

Developmental diversification of cortical inhibitory interneurons

PRJNA411832 | ENA

Autophagy is dispensable for the maintenance of parvalbumin interneurons but required for their inhibitory neurotransmission and memory

Previous studies have demonstrated the requirement of autophagy for neuronal survival and synaptic function, mostly focusing on projection neurons and excitatory neurotransmission. Here, we examine the effects of conditional atg5 ablation in neurons expressing parvalbumin (PV-cKO), which in the fore...

ORGANISM(S): Mus musculus (Mouse)

2026-02-02 | PXD035264 | Pride

Autophagy Mus musculus Neurons Fusion

brpf1 knockdown reduces inhibitory neurotransmission and regulates gene expression of GABAergic interneurons derived from MGE

PRJNA663318 | ENA

Occupancy and transcriptional profile of Prdm12 in posteriorized neural tissue

V1 interneurons are a class of inhibitory neurons that play an essential role in vertebrate locomotion; however, the factors contributing to their specification are poorly understood. Morphological and molecular evidence suggest that the zinc finger transcription factor Prdm12 may play a role in pro...

ORGANISM(S): Xenopus laevis

2015-11-04 | E-GEOD-64551 | biostudies-arrayexpress

Prefrontal D2- dopamine receptor deactivates top-down inhibitory control by regulating striatal cholinergic interneurons

PRJNA983850 | ENA

Nr4a1 regulates cell-specific transcriptional programs in inhibitory GABAergic interneurons

The patterns of synaptic connectivity and physiological properties of diverse neuron types are shaped by distinct gene sets. Our study demonstrates that, in the mouse forebrain, the transcriptional profiles of inhibitory GABAergic interneurons are regulated by Nr4a1, an orphan nuclear receptor whose...

ORGANISM(S): Mus musculus

2024-06-05 | GSE263082 | GEO

Activity-dependent gene regulations in PV+ interneurons

PRJNA924748 | ENA

Development and Diversification of Retinal Amacrine Interneurons at Single Cell Resolution

The vertebrate retina uses diverse neuronal cell types arrayed into complex neural circuits to extract, process and relay information from the visual scene to the higher order processing centers of the brain. Amacrine cells, a diverse class of inhibitory interneurons, are thought to mediate the maj...

ORGANISM(S): Mus musculus

2009-05-31 | E-GEOD-12601 | biostudies-arrayexpress

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