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TranscriptomicsGeneration of Cloned Mice from Adult Neurons by Direct Nuclear Transfer
Whereas cloning mammals by direct somatic cell nuclear transfer has been successful using a wide range of donor cell types, neurons from adult brain remain M-bM-^@M-^\unclonableM-bM-^@M-^] for unknown reasons. Here we examined whether neurons from adult mice could be cloned, using a combination of t...
ORGANISM(S): Mus musculus 
2015-02-03 | E-GEOD-65497 | biostudies-arrayexpress
TranscriptomicsDonor cell type-specific gene expression in embryonic but not extraembryonic tissues of postimplantation embryos cloned from somatic cells
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2013-09-01 | E-GEOD-49173 | biostudies-arrayexpress
TranscriptomicsRNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryo
Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient. Most SCNT-generated embryos die after implantation because of unidentified, complex epigenetic errors in the process of postimplantation embryonic development. Here, we identified the most upstream level of dysfunction le...
ORGANISM(S): Mus musculus 
2011-11-17 | E-GEOD-33208 | biostudies-arrayexpress
TranscriptomicsHistone chaperone CAF-1 is essential for retrotransposon silencing by mediating histone H4K20me3 deposition in mouse preimplantation embryos
Retrotransposons are widely spread in the mammalian genome and are usually silenced during development to avoid transposition-inducing mutations. But how they are repressed in embryos shortly before implantation remain to be identified, since the genome at this stage is globally hypomethylated. Here...
ORGANISM(S): Mus musculus 
2015-11-01 | E-GEOD-69260 | biostudies-arrayexpress
TranscriptomicsComparative gene expression analyses using E4.0 cloned blastocysts
The great majority of embryos generated by somatic cell nuclear transfer (SCNT) display defined abnormal phenotypes after implantation, such as an increased likelihood of death and abnormal placentation. To gain better insight into the underlying mechanisms, we analyzed genome-wide gene expression p...
ORGANISM(S): Mus musculus 
2013-09-01 | E-GEOD-49172 | biostudies-arrayexpress
TranscriptomicsComparative gene expression analyses using post-implanted E6.5 cloned embryos
The great majority of embryos generated by somatic cell nuclear transfer (SCNT) display defined abnormal phenotypes after implantation, such as an increased likelihood of death and abnormal placentation. To gain better insight into the underlying mechanisms, we analyzed genome-wide gene expression p...
ORGANISM(S): Mus musculus 
2013-09-01 | E-GEOD-49171 | biostudies-arrayexpress
TranscriptomicsTrichostatin A specifically improves the aberrant expression of transcription factor genes in embryos produced by somatic cell nuclear transfer
Although mammalian cloning by somatic cell nuclear transfer (SCNT) has been established in various species, the low developmental efficiency has hampered its practical applications. Treatment of SCNT-derived embryos with histone deacetylase (HDAC) inhibitors can improve their development, but the un...
ORGANISM(S): Mus musculus 
2015-04-01 | E-GEOD-63886 | biostudies-arrayexpress
TranscriptomicsImpeding Xist expression from the active X chromosome improves mouse somatic cell cloning
Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient because of aberrant genomic reprogramming. In addition to random reprogramming errors, we hypothesized the presence of specific errors as evidenced by common anomalies among clones. We found that Xist, which normally inact...
ORGANISM(S): Mus musculus 
2010-09-16 | E-GEOD-23181 | biostudies-arrayexpress
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