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MetabolomicsGlobal 13C tracing and metabolic flux analysis of intact human liver tissue ex vivo
Liver metabolism is central to human physiology and influences the pathogenesis of common metabolic diseases. Yet, our understanding of human liver metabolism remains incomplete, with much of current knowledge based on animal or cell culture models that do not fully recapitulate human physiology. He...
2024-07-23 | MTBLS10481 | MetaboLights
MetabolomicsHidden molecular clues in marine sediments revealed by untargeted mass spectrometry imaging

Mass spectrometry imaging (MSI) in sedimentary archives can produce records of molecular proxies at μm-scale resolution. For example, in annually varved sediments of the Santa Barbara Basin, such a fine resolution allows deciphering sub-annual distributions of archaeal tetraether lipids, haptophy...

2022-08-15 | MTBLS4609 | MetaboLights
MetabolomicsMass spectrometry imaging of phosphatidylcholine metabolism in lungs administered with therapeutic surfactants and isotopic tracers

Mass spectrometry imaging (MSI) visualises molecular distributions throughout tissues but is blind to dynamic metabolic processes. Here, MSI with high mass resolution together with multiple stable isotope labelling provided spatial analyses of phosphatidylcholine (PC) metabolism in mouse lungs. D...

2020-10-30 | MTBLS2075 | MetaboLights
ProteomicsGlycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis
Glycosylation is an important post-translational modification (PTM) mechanism of proteins, that modulates protein function. Measurement of clinically adopted cancer protein biomarkers is commonly performed using affinity-based techniques, which often suffer from poor specificity and inability to dis...
ORGANISM(S): Homo sapiens (Human) 
2023-01-11 | PXD038602 | Pride
Intact proteins Hepatocellular carcinoma 
MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
ProteomicsGlycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis
Alpha fetoprotein (AFP) is a circulating cancer biomarker implicated in multiple neoplastic malignancies, including hepatocellular carcinoma (HCC). AFP glycoforms with core fucosylation structure (AFP-L3) are used clinically as a biomarker, to predict risk of developing HCC and to monitor its recur...
ORGANISM(S): Homo sapiens (Human) 
2023-01-11 | PXD038606 | Pride
Afp-l3 proteforms Core fucose Fucosylation High-resolution mass spectrometry Intact protein analysis 
MetabolomicsElucidation of xenoestrogen metabolism by non-targeted, stable isotope-assisted mass spectrometry in breast cancer cells

Environmental exposure to xenoestrogens, i.e., chemicals that imitate the hormone 17β-estradiol, has the potential to influence hormone homeostasis and action. Detailed knowledge of xenobiotic biotransformation processes in cell models is key when transferring knowledge learned from in vitro<...

2023-10-26 | MTBLS2716 | MetaboLights
UnknownBest practices and benchmarks for intact protein analysis for top-down mass spectrometry.
Not available
S-EPMC6719561 | biostudies-literature
ProteomicsIntact mass LC-MS analysis of Flu B HA1 and HA2 proteins
To determine the sequences of the Flu HA1 and HA2 proteins, we performed intact mass LC-MS analysis. By reducing disulfide bridges two peaks corresponding to the HA1 and HA2 components were detected. Mass profiles were obtained for both glycosylated and deglycosylated HA proteins.
ORGANISM(S): Influenza A virus (A/Aquatic bird/Hong Kong/M603/98(H11N1)) 
2025-05-06 | PXD046130 | Pride
Intact mass Ha2 Ha1 Flu b 
ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
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