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MetabolomicsThe Impacts of Sortase A and the 4'-Phosphopantetheinyl Transferase Homolog Sfp on Streptococcus mutans Extracellular Membrane Vesicle Biogenesis

Extracellular membrane vesicles (EMVs) are produced by many Gram-positive organisms, but information regarding vesiculogenesis is incomplete. We used single gene deletions to evaluate the impacts on Streptococcus mutans EMV biogenesis of Sortase A (SrtA), which affects S. mutans...

2021-10-06 | MTBLS1812 | MetaboLights
MetabolomicsA multi-omics approach reveals functions of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees
Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved proteins that are involved in membrane trafficking. An integrated approach was taken to elucidate function of SCAMPs in wood formation of Populus trees. The metabolomics analyses were performed in woody tissues of transgeni...
2018-01-08 | MTBLS478 | MetaboLights
MetabolomicsIntegrated proteome and lipidome analyses place OCIAD1 at mitochondria-peroxisome intersection balancing lipid metabolism

OCIAD1 (Ovarian Cancer Immunoreactive Antigen Domain Containing 1) is a membrane protein largely localized to mitochondria, however, its function in health or disease is not well understood. To comprehensively characterize the molecular changes upon lack of OCIAD1, we used mass spectrometry to st...

2025-04-25 | MTBLS12329 | MetaboLights
ProteomicsMembrane proteome of HEK293T cells
We developed a simple and effective method for “in-depth” membrane proteomics. We optimize the sample preparation for the maximum numbers of the identified membrane proteins using a human cell line, HEK293T, as a model. From this study, we demystified the merits of Urea wash methods for membrane enr...
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027473 | JPOST Repository
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-02 | MSV000080014 | MassIVE
ProteomicsThe Whole cell lysate and Crude membrane of HEK293T cells
We perform the experiment in order to compare identified membrane proteins from whole cell lysate and membrane fraction in HEK293T cell. The isolation of membrane fractions is critical to obtain a more comprehensive membrane proteome, especially with proteins containing transmembrane domains.
ORGANISM(S): Homo Sapiens (human) 
2022-04-28 | PXD027472 | JPOST Repository
ProteomicsMembrane proteome of renal BBMVs from the mouse model
In order to demonstrate the efficacy of the membrane enrichment method by Urea Wash in the animal model. Kidneys were dissected from 8 weeks old male mice of inbred strain C57BL/6J and flash-frozen in liquid nitrogen followed by storage at -80 °C. Brush border membrane vesicles (BBMV) were prepared...
ORGANISM(S): Mus Musculus (mouse) 
2022-04-28 | PXD027474 | JPOST Repository
ProteomicsTowards a quantitative assessment of the integral magnetosome membrane sub-proteome
Magnetosomes are membranous organelles than contain crystals of magnetic minerals and enable magnetotactic bacteria to orientate in magnetic fields. The enveloping magnetosome membrane was found to contain a subset of specific proteins that are encoded within a genomic island. However, along with pr...
ORGANISM(S): Magnetospirillum gryphiswaldense 
2017-10-24 | PXD006166 | Pride
Organelle Membrane proteome Magnetosome 
ProteomicsProfiling antibody responses to M. tuberculosis proteins in TB suspects’ sera using full-proteome microarrays.
Understanding the immune response to tuberculosis requires greater knowledge of humoral responses. To characterize antibody targets and the effect of disease parameters on target recognition, we developed a systems immunology approach that integrated detection of antibodies against the entire Mycob...
ORGANISM(S): Homo sapiens 
2010-07-13 | E-GEOD-19433 | biostudies-arrayexpress
ProteomicsMembrane proteomis profile along human colon
The colonic epithelium is a highly dynamic system important for regulation of ion and water homeostasis via absorption and secretion, and in maintaining a protective barrier between the outer milieu and the inside of our body. These processes are known to gradually change along the length of the col...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-07 | MSV000079561 | MassIVE
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