Interneuron - OmicsDI

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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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Lab affiliation

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Department of Cell Biology, Emory University School of Medicine, Atlanta, GA, USA (1)
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, Italy (1)
Department of Fundamental Neurosciences (DNF) University of Lausanne Rue du Bugnon 9 CH-1005 Lausanne Switzerland (1)
Developmental Neurobiology, Max Planck Institute of Psychiatry, Munich, Germany (1)
Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Germany (1)
MRC Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom (1)
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University of Basel, Biozentrum, Switzerland (1)
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Isl1-Lhx3 fusion specifies motor neuron fate by inducing motor neuron genes and concomitantly suppressing the interneuron programs

Combinatorial transcription codes generate the myriad of cell types during development, and thus likely provide crucial insights into directed differentiation of stem cells to a specific cell type. The LIM-complex composed of Isl1 and Lhx3 directs the specification of spinal motor neurons (MNs) in e...

ORGANISM(S): Mus musculus

2012-02-03 | E-GEOD-35510 | biostudies-arrayexpress

Cell-intrinsic regulation of interneuron migration controls cortical neurogenesis

Interneurons navigate along multiple tangential paths to settle into appropriate cortical layer. They undergo saltatory migration, which is paced by intermittent nuclear jumps whose regulation relies on interplay between extracellular cues and genetic-encoded information. However, it remains unclear...

ORGANISM(S): Mus musculus (Mouse)

2018-02-14 | PXD006397 | Pride

Comparative analysis of different cortical interneuron groups.

GABAergic interneuron in the cortex comprise a very heterogenous group. and it is critical to identify discrete interneuron types to understand how their contributions to behavior can be modulated by external and internal cues. However, molecular difinition of these interneuron cell groups has been ...

ORGANISM(S): Mus musculus

2014-10-07 | E-GEOD-56996 | biostudies-arrayexpress

Trisomy 21 human iPSC derived interneuron progenitors

PRJNA832126 | ENA

Sip1 in cortical interneuron migration

We sequenced mRNA from 6 samples of FACsorted telencephalons from E14.5 Sip1|Nkx2-1 knockout and WT|Nkx2-1 control mouse embryos to find differentially expressed genes in the absence of the transcription factor Sip1. Examination of mRNA levels in 3 control and 3 Sip1|Nkx2-1 knockout samples

ORGANISM(S): Mus musculus

2012-12-07 | E-GEOD-35616 | biostudies-arrayexpress

Fate plasticity of interneuron specification

PRJNA1167741 | ENA

Roles of Arx in developing interneuron [scRNA-seq]

PRJNA1145447 | ENA

Roles of Arx in developing interneuron [ChIP-seq]

PRJNA1145448 | ENA

Pyramidal neurons proportionately alter the identity and survival of specific cortical interneuron subtypes

PRJNA1138229 | ENA

Short- and long-distance extrinsic regulation of interneuron specification and migration

PRJNA837579 | ENA