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Invitro assay to look at phosphorylation of esp8 by NleH1/2 compared to KED mutant
ORGANISM(S): Escherichia coli 
2022-08-07 | PXD010925 | Pride
High-throughput single-cell mitochondrial DNA genotyping reveals properties of clonal variation in human contexts [CD34-invitro]
Aff4 was recently identified as an insulin-regulated phosphoprotein. To investigate upstream kinases, we analysed immunoprecipitated Aff4 treated with our without insulin and treated with either an Akti or a P70S6Ki. These results showed that AFF4 is dependent on P70S6K and therefore an in vitro kin...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-16 | PXD054247 | Pride
The goal was to obtain the differential transcriptome in the deep cones fibroblasts between shallow and deep wounds in Duroc breeds at 20 wks. We made shallow and deep wounds on the backs 2 Duroc pigs, biopsied the wounds at 20 weeks, cultured fibroblasts, extracted and the RNA from the cultured fi...
ORGANISM(S): Sus scrofa 
Neutrophils are key effector cells of the innate immune response and are required to migrate and function within highly adverse micro-environmental conditions. These inflammatory sites are characterised by low levels of oxygen. We examined the relative transcript abundance detected in an apoptosis...
ORGANISM(S): Homo sapiens 
Human Peripheral blood mononuclear cells were purified from older volunteers' blood and were cultured for 24 hrs in the presence or abscence of angiotensin type 1 receptor autoantibody (AT1RaAb 7ug/ml). In brief: PBMCs were isolated from heparinized venous blood by Ficoll-Hypaque density gradient ce...
ORGANISM(S): Homo sapiens 
2018-02-25 | GSE66331 | GEO
We used microarrays to detail the global gene expression of mithramycin treated cells, xenografts and sp1 depleted cells with p53 overexpression. MPM cells and xenografts were treated with mithramycin or SP1 depleted/p53 overexpressed MPM cells were selected for RNA extraction and hybridization on A...
ORGANISM(S): Homo sapiens 
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