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MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
MetabolomicsMass spectrometry imaging as an emerging tool for studying metabolism in human brain organoids

Human brain organoids are emerging models to study human brain development and pathology as they recapitulate the development and characteristics of major neural cell types, and enable manipulation through an in vitro system. Over the past decade, with the advent of spatial technologies,...

2023-05-23 | MTBLS7565 | MetaboLights
ProteomicsHERD-1 Flag IP mass data in C.elegans
Proteins that may interact with HERD-1 were identified with Flag IP-mass in animals, which were tagged with 3xflag::GFP before the stat condon of HERD-1 in C.elegans.
ORGANISM(S): Caenorhabditis elegans 
2024-07-14 | PXD051885 | Pride
Herd-1 Ip mass C.elegans 
ProteomicsSILAC IP-Mass spectrometry of TTLL-transfected HEK cells
Identification of the non-tubulin substrates of tubulin glycylases, TTLL3/TTLL8 and tubulin glutamylases TTLL4/TTLL5 using SILAC-IP-MS/MS quantitative mass spectrometry approach. HEK cells transfected with the respective enzymes were lysed and the proteins modified by the enzymes enriched by immunop...
ORGANISM(S): Homo sapiens (Human) 
2026-05-18 | PXD065251 | Pride
Hek cells Ttlls Ip-ms/ms Silac 
Proteomicsimmunoprecipitation-mass spectrometry (IP-MS) of FDR-1
immunoprecipitation-mass spectrometry (IP-MS)of FDR-1
ORGANISM(S): Caenorhabditis elegans 
2025-08-07 | PXD066847 | Pride
Fdr-1 ip-ms 
ProteomicsDiscovery of Klf2 Interactors in Mouse Embryonic Stem Cells by Immunoprecipitation-Mass Spectrometry Utilizing Exogenously Expressed Bait
Krüppel-like factor 2 (Klf2) is a DNA-binding transcription factor that regulates embryonic stem cell-specific gene expression. Transcription cofactors such as p300 acetyltransferases and Erk kinases interact with Klf2, providing an additional layer of transcription regulation in embryonic stem cell...
ORGANISM(S): Mus Musculus (mouse) 
2021-09-20 | PXD022123 | JPOST Repository
ProteomicsAc-IP tag enables multiplexed proteome quantification in data-independent acquisition mode
We propose the isotopically labeled Ac-IP (acetyl-isoleucine-proline) tag for multiplexed quantification of peptides at the MS1 level in the data-independent acquisition (DIA) mode. Differentially labeled peptides have distinct precursor ions carrying the quantitative information but identical MS2 s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-07 | PXD022606 | Pride
Quantitative proteomics Isotope labeling Data-independent acquisition 
TranscriptomicsNext-generation sequencing for the fat mass and obesity-associated protein (FTO) binding mRNA
Purpose: The goals of this study are to investigate the mRNAs that bind to the FTO protein in the white fat tissue from mice. Methods: 1. White fat from CAG promoter driven transgenic Flag-tagged FTO mice were extracted with RNA protected. 2. Flag-FTO proteins were immunoprecipitated with control I...
ORGANISM(S): Mus musculus 
2016-01-05 | E-GEOD-74255 | biostudies-arrayexpress
ProteomicsEndo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons
We developed a toolkit for capturing endosomes (Endo-IP) and lysosomes (Lyso-IP) in human stem cell(hESC)-derived induced neurons (iNeurons). We target a sub-population of early endosomes marked by endogenously tagged EEA1 (Early Endosome Antigen 1) protein, which represents one of the earliest popu...
ORGANISM(S): Homo sapiens (Human) 
2025-01-11 | PXD055978 | Pride
Human Tmt Endosomes Ineurons Lysosomes 
UnknownThe Isotopic Ac-IP Tag Enables Multiplexed Proteome Quantification in Data-Independent Acquisition Mode.
Not available
S-EPMC8209779 | biostudies-literature
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