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ProteomicsMass spectrometry-based analysis of Mycobacterial single-colony proteome
Here as an attempt to explore bacterial single-colony proteomics, we describe a quantitative mass spectrometry-based protocol to isolate and analyse the proteome of a single mycobacterial colony from 7H10 media. Tryptic peptides are evaluated with high performance liquid chromatography coupled with ...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2021-03-11 | PXD018168 | Pride
Protein extraction Mycobacteria Bacterial proteome Mass spectrometry Single-colony Proteomics 
ProteomicsComparison between the proteome of Escherichia coli single colony and during liquid culture
Most of the proteomic studies done so far uses bacterial cells harvested from liquid culture media. However it is widely accepted that many important determinants associated with virulence and host cell adhesion are exclusively expressed during growth on solid media. As an attempt to complement prev...
ORGANISM(S): Escherichia coli 
2021-09-09 | PXD019140 | Pride
Protein extraction Bacterial proteome Ecoli Mass spectrometry Single-colony Proteomics 
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