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ProteomicsFree-Flow Isoelectric Focusing for Comprehensive Separation and Analysis of Human Salivary Microbiome for Lung Cancer
Human microbiome contains billions of microorganisms that play important roles in the biological system and different diseases. Due to its complexity, conventional culture-independent technology may underestimate the value of low-abundance bacteria, which calls for a highly efficient method for its ...
ORGANISM(S): Homo Sapiens 
2021-03-10 | PXD024619 |
ProteomicsSystematic Discovery of Deeply Conserved Plant Protein Complexes, Broccoli nucleii, isoelectric focusing
The associated files are mass spec data from native nuclear extract from broccoli florets (Brassica oleracea var. italica)fractionated on an Agilent 3100 OFFGEL isoelectric focusing apparatus.
ORGANISM(S): Brassica oleracea var. italica 
2019-10-28 | PXD013281 | Pride
Broccoli Isoelectric focusing Ief Nuclear extract Cf-ms Offgel Brassica oleracea 
ProteomicsSystematic Discovery of Deeply Conserved Plant Protein Complexes, Wheat germ Isoelectric focusing
The associated files are mass spec data from an isoelectric focusing separation of native wheat (Triticum aestivum) germ extract. The non-denaturing separation was achieved using an Agilent 3100 OFFGEL Fractionation apparatus.
ORGANISM(S): Triticum aestivum (Wheat) 
2019-10-28 | PXD013280 | Pride
Triticum aestivum Ief Isoelectric focusing Wheat germ Cf-ms Offgel Cofractionation mass spec 
ProteomicsAutomated capillary isoelectric focusing-tandem mass spectrometry for top-down proteomics
Mass spectrometry (MS)-based top-down proteomics (TDP) delineates proteoforms in cells and requires high-resolution proteoform separation. Herein, for the first time, we employed an automated capillary isoelectric focusing-tandem MS (cIEF-MS/MS) system for large-scale TDP of complex proteomes. Singl...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Escherichia coli 
2020-12-08 | PXD020342 | Pride
Endogenous hormone proteoforms/gel-free 2d-page/multi-level proteomics/ sexual dimorphism/zebrafish brain 
UnknownCapillary isoelectric focusing of individual mitochondria.
Not available
S-EPMC3021621 | biostudies-literature
UnknownCascaded free-flow isoelectric focusing for improved focusing speed and resolution.
Not available
S-EPMC2560584 | biostudies-literature
UnknownIsoelectric focusing technology quantifies protein signaling in 25 cells.
Not available
S-EPMC1618307 | biostudies-literature
UnknownArrayed isoelectric focusing using photopatterned multi-domain hydrogels.
Not available
S-EPMC6106862 | biostudies-literature
UnknownBistable isoelectric point photoswitching in green fluorescent proteins observed by dynamic immunoprobed isoelectric focusing.
Not available
S-EPMC3488114 | biostudies-literature
ProteomicsFree-Flow Isoelectric Focusing Based Metaproteomics Workflow Facilitates the Identification and Characterization of Human Salivary Microbiome for Lung Cancer
We developed a free-flow isoelectric focusing (FFIEF) based metaproteomics workflow to reduce the host interferences and enrich the low-abundant bacteria for better interpretation of salivary microbiome. We firstly tested the pretreatment module that could significantly reduce the host interferences...
ORGANISM(S): human saliva metagenome 
2022-11-17 | PXD026727 | Pride
Taxonomy-function integration Lung cancer Metaproteomics Free-flow isoelectric focusing Salivary microbiome Dysbiosis 
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