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MetabolomicsProteomic analysis validates previous findings on wounding-responsive plant hormone signaling and primary metabolism contributing to the biosynthesis of secondary metabolites based on metabolomic analysis in harvested broccoli (Brassica oleracea L. var. italica)
The plant wound-response is a complex process that generates physiological modifications for protecting the wounded tissue. In this study, tandem mass tag (TMT)-based quantitative proteomic analysis was performed to clarify the comprehensive molecular mechanism for the wound-response of broccoli subj...
2021-05-19 | MTBLS2317 | MetaboLights
TranscriptomicsExpression data from 11-day-old antisense BoEMF2.1 broccoli
EMF2 genes play an important function in inhibiting reproductive programs during vegetative stage of plant development. We knocked down the expression of endogenous EMF2 genes in broccoli by introduing a 35S::antisenseBoEMF2.1 contruct. The transgenic broccoli with knocking-down expression of endoge...
ORGANISM(S): Brassica oleracea var. italica 
2012-03-30 | E-GEOD-36963 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human colon Caco2 cells treated with extracts from broccoli that had been cooked for different lengths of time
Caco2 cells were treated 24 hours with broccoli extracts that had been cooked for different lengths of time.
ORGANISM(S): Homo sapiens 
2008-09-30 | E-MEXP-1372 | biostudies-arrayexpress
TranscriptomicsIdentification of miRNAs-seq of 4-day broccoli sprouts and putative cross-kingdom regulated human target genes
We want to obtained miRNA-seq from 4 day-old broccoli sprouts by deep sequencing and identified the binding sites of the those miRNAs in human target genes. Meanwhile, we extracted the human target genes from published paper that were regulated by broccoli and compared those genes with the predicte...
ORGANISM(S): Brassica rapa 
2020-02-01 | E-MTAB-7155 | biostudies-arrayexpress
TranscriptomicsTranscriptional analysis of exogenously supplied and transgenic of senescence-induced cytokinin for broccoli postharvest yellowing improvement
Both exogenously supplied and transgenic induced cytokinin production can effectively delay senescence of broccoli florets during postharvest storage. However, a substantial comparison between the mechanisms of these two treatments on delaying broccoli florets senescence was absent. Here, we conduct...
ORGANISM(S): Brassica oleracea var. italica 
2013-05-01 | E-GEOD-42609 | biostudies-arrayexpress
TranscriptomicsIdentification and comparative analysis of miRNAs and their targets associated with curd-forming capacity at high temperature in two broccoli genotypes
We report miRNA expression profiles underlying curd-forming capacity at high temperature in two broccoli lines by high-throughput sequencing. Examination of miRNA expression profiles in 2 broccoli isogenic lines with different curd-forming capacity at high temperature (High curd-forming capacity: ...
ORGANISM(S): Brassica oleracea var. italica 
2014-09-03 | E-GEOD-50546 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of human prostate from patients with a previous diagnosis of Prostatic Intraepithelial Neoplasia and following consumption of high glucosinolate broccoli or peas to investigate interactions with the GSTM1 genotype
Twenty-two male volunteers aged 30-70 years with a previous diagnosis of Prostatic Intraepithelial Neoplasia (PIN) were recruited by a consultant urologist at Norfolk and Norwich University Hospital NHS Trust. Ethical approval for the trial was obtained from the Norfolk Research Ethics Committee (re...
ORGANISM(S): Homo sapiens 
2008-07-16 | E-MEXP-1243 | biostudies-arrayexpress
ProteomicsSystematic Discovery of Deeply Conserved Plan Protein Complexes, Broccoli leaves
The associated files are mass spec data from individual fractions of mixed-bed ion exchange fractionations of native extract made from broccoli plant leaves (Brassica oleracea var. italica).
ORGANISM(S): Brassica oleracea var. italica 
2019-10-28 | PXD013282 | Pride
Broccoli Leaves Iex Ion exchange Brassica oleracea var. italica Cf-ms 
ProteomicsSystematic Discovery of Deeply Conserved Plant Protein Complexes, Broccoli nucleii, isoelectric focusing
The associated files are mass spec data from native nuclear extract from broccoli florets (Brassica oleracea var. italica)fractionated on an Agilent 3100 OFFGEL isoelectric focusing apparatus.
ORGANISM(S): Brassica oleracea var. italica 
2019-10-28 | PXD013281 | Pride
Broccoli Isoelectric focusing Ief Nuclear extract Cf-ms Offgel Brassica oleracea 
ProteomicsSystematic Discovery of Deeply Conserved Plant Protein Complexes, Broccoli Nuclei
The associated files are mass spec data from individual fractions of ion exchange or size exclusion fractionations of native nuclear extract made from broccoli florets (Brassica oleracea var. italica). Ion exchange chromatography was on a concatenated WAX-WAX-CAT column series.
ORGANISM(S): Brassica oleracea var. italica 
2019-10-28 | PXD013322 | Pride
Ion exchange Broccoli Brassica oleracea var. italica Cf-ms Size exclusion Nuclei 
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