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UnknownDextrose-mediated aggregation of therapeutic monoclonal antibodies in human plasma: Implication of isoelectric precipitation of complement proteins.
Not available
S-EPMC4966494 | biostudies-literature
ProteomicsImproving fractionation of human milk proteins through calcium phosphate co-precipitation and their rapid characterization by capillary electrophoresis
This work describes a simple sample pretreatment method for the fractionation of human milk proteins (into their two main groups: whey and caseins) prior to their analysis. The protein extraction protocol is based on the addition of calcium phosphate to non-adjusted pH human milk. The combination of...
ORGANISM(S): Homo sapiens (Human) 
2018-08-31 | PXD010315 | Pride
Calcium phosphate fractionation  human milk whey proteins  human milk caseins  capillary electrophoresis  fraction collection 
UnknownStructural and functional properties of collagen isolated from lumpfish and starfish using isoelectric precipitation vs salting out.
Not available
S-EPMC10053375 | biostudies-literature
UnknownEffect of ultrasound on the functional properties and structural changes of chicken liver insoluble proteins isolated by isoelectric solubilization/precipitation.
Not available
S-EPMC11634992 | biostudies-literature
UnknownEffective Decolorization of Poly-γ-Glutamic Acid Fermentation Broth by Integrated Activated Carbon Adsorption and Isoelectric Point Precipitation of Glutamic Acid.
Not available
S-EPMC11643718 | biostudies-literature
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture protein aggregates, ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2022-08-01 | PXD032095 | Pride
Microparticles Proteomics Sample preparation Protein aggregation Contaminant removal Lc−ms/ms Protein precipitation 
UnknownIsoelectric point-amyloid formation of α-synuclein extends the generality of the solubility and supersaturation-limited mechanism.
Not available
S-EPMC8244297 | biostudies-literature
TranscriptomicsMethylation profiling in various human cell lines and tissues by mDIP - methylated DNA precipitation with antibodies against methylated cytosine
Finding the methylation state of DNA promoters in various cell lines and tissues by mDIP - methylated DNA precipitation with antibodies against methylated cytosine.
ORGANISM(S): Homo sapiens 
2006-02-01 | E-MEXP-520 | biostudies-arrayexpress
ProteomicsFree-Flow Isoelectric Focusing Based Metaproteomics Workflow Facilitates the Identification and Characterization of Human Salivary Microbiome for Lung Cancer
We developed a free-flow isoelectric focusing (FFIEF) based metaproteomics workflow to reduce the host interferences and enrich the low-abundant bacteria for better interpretation of salivary microbiome. We firstly tested the pretreatment module that could significantly reduce the host interferences...
ORGANISM(S): human saliva metagenome 
2022-11-17 | PXD026727 | Pride
Taxonomy-function integration Lung cancer Metaproteomics Free-flow isoelectric focusing Salivary microbiome Dysbiosis 
ProteomicsA Comparative Proteomic Analysis of Whey Protein from Early, Mid and Late Lactation Stages of Indian Indigenous Breed, MalnadGidda (Bosindicus)
Different lactation stages have marked influence on milk yield, milk constituents and nourishment of the neonates. However, the differential gene expression during different lactation stages in Bosindicus has not been investigated so far. In this study, we carried out high-resolution mass spectromet...
ORGANISM(S): Bos indicus 
2018-02-16 | PXD007655 | Pride
Ultracentrifugation Tmt labeling Isoelectric precipitation Brplc Biomarker 
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