Sort   by:  
 Page size 
MetabolomicsMetabolomic Characterization of Knock-Out Mutants in Arabidopsis - Development of a Metabolite Profiling Database for Knock-Out Mutants in Arabidopsis (MeKO)
Despite recent intensive research efforts in functional genomics, the functions of only a limited number of Arabidopsis (Arabidopsis thaliana) genes have been determined experimentally. Thus, improving gene annotation remains a major challenge in plant science. Because metabolite profiling can chara...
2014-03-01 | MTBLS47 | MetaboLights
ProteomicsMMV2224 project isothermal TPP experiments
Isothermal TPP was performed essentially as previously described (Milne, R., et al., Toolkit of Approaches To Support Target-Focused Drug Discovery for Plasmodium falciparum Lysyl tRNA Synthetase. ACS Infect Dis, 2022. 8(9): p. 1962–1974). In this instance P. falciparum lysates incubated ± drug were...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2026-09-07 | PXD075163 | Pride
Isothermal proteome profiling Mmv22224 Plasmodium falciparum 
MetabolomicsWax ester and lipophilic compound profiling of Euglena gracilis by gas chromatography-mass spectrometry: toward understanding of wax ester fermentation under hypoxia
Lipids are being increasingly used as biodiesel feedstock, and several saturated wax esters from Euglena gracilis are candidates for outdoor bulk production. Wax ester fermentation in Euglena is strongly increased by hypoxia, but key events underlying the metabolic shift toward wax ester biosynthesi...
2014-09-04 | MTBLS113 | MetaboLights
UnknownAn isothermal shift assay for proteome scale drug-target identification.
Not available
S-EPMC7021718 | biostudies-literature
ProteomicsThermal Proteome Profiling Datasets for smarTPCA
Chemical biology and the application of small molecules has proven to be a potent perturbation strategy especially for the functional elucidation of proteins, their networks and regulators. In recent years, the cellular thermal shift assay (CETSA) and its proteome-wide extension, thermal proteome pr...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD032942 | Pride
Kinase inhibitors Small molecule arranged thermal proximity coaggregation (smartpca) Tpp with temperature range (tpp-tr) or compound concentration range (tpp-ccr) Hl-60 cell line Thermal proteome profiling (tpp) 
ProteomicsDetermine PARP1 and PARP2 target engagment of Niraparib and Olaparib in live cells
Determine the target engagment of PARP inhibitors (Niraparib and Olaparib) to PARP1 and PAPR2 in live cells using isothermal dose-response experiments
ORGANISM(S): Homo sapiens (Human) 
2025-05-13 | PXD044332 | Pride
Parp2 Parp1 Tpp Niraparib Itdr Olaparib 
UnknownIon-Based Proteome-Integrated Solubility Alteration Assays for Systemwide Profiling of Protein-Molecule Interactions.
Not available
S-EPMC9118197 | biostudies-literature
ProteomicsRapid discovery of drug target engagement by isothermal shift assay
Determining protein targets directly bound by drug molecules remains challenging. Here we present the isothermal shift assay, iTSA, for rapid unbiased identification of drug targets. Compared with thermal proteome profiling, the prevailing method for target engagement, iTSA offers a simplified workf...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-28 | MSV000083640 | MassIVE
ProteomicsIon-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions
Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity over the last several years. However, these methods have limi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD030695 | Pride
Proteomics Mechanism of action Drug target deconvolution Thermal protein profiling Cancer 
ProteomicsIon-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions - full curve data
Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity over the last several years. However, these methods have limi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD033081 | Pride
Proteomics Mechanism of action Drug target deconvolution Thermal protein profiling Cancer 
Sort   by:  
 Page size