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ProteomicsMass spectrometric ITEM-TWO analyses of interactions between human cardiac Troponin T (hcTnT) peptides and the M7 or the M11.7 antiTnT antibodies.
Molecular characterization of antibodies M7, and M11.7, as well as the antiTNF alpha antibody in addition to the synthetic peptides P11-P17 and P21-P32. The M7 and M11.7 anti-human cardiac Troponin T (antihcTnT) antibodies from Roche Diagnostics Deutschland GmbH (Mannheim, Germany) and the antiTNF a...
ORGANISM(S): Homo sapiens (Human) 
2025-05-20 | PXD058812 | Pride
Item-two Troponin t Offline nanoesi-ms 
ProteomicsMass spectrometric ITEM-ONE analysis confirms and refines an assembled affimer binding site on the HER-2 targeting therapeutic antibody Pertuzumab
The Pertuzumab-binding affimer 00557_709097 was incubated either with peptides from tryptically digested Pertuzumab or with selected synthesized peptides. Affimer-peptide complexes were subjected to nano electrospray mass spectrometric ITEM analyses in order to identify the affimer’s epitope on Pert...
ORGANISM(S): Homo sapiens (Human) 
2024-04-09 | PXD043203 | Pride
Pertuzumab Item Affimer Offline nanoesi-ms 
ProteomicsIntact Transition Epitope Mapping- Thermodynamic Weak-force Order (ITEM-TWO)
Most important characteristics of antibodies are that they typically strongly bind to specific epitopes, thereby expressing low dissociation constants (KDs) and high Gibbs free binding energies (delta Gs). In this study, we describe an off-line nano-electrospray mass spectrometry method, termed IT...
ORGANISM(S): Homo sapiens (Human) 
2019-11-07 | PXD016024 | Pride
Antibody affinity determination Antibody specificity determination 
ProteomicsMass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization
We describe theoretical concepts and explain practical procedures of an electrospray mass spectrometry method, termed “Intact Transmission Epitope Mapping – Thermodynamic Weak-force Order (ITEM-TWO)”, by which can be determined apparent binding energies and dissociation constants of immune complex ...
ORGANISM(S): Homo sapiens (Human) Equus caballus (Horse) 
2020-10-29 | PXD021296 | Pride
Protocol Item-two Rnase s Offline esi-ms Troponin i epitope peptide-antitroponin i antibody complex Myoglobin Flag-peptide–antiflag antibody complex 
ProteomicsMass spectrometry-guided molecular modeling predicts the interaction interface for a camelid single-domain antibody targeting the Plasmodium falciparum circumsporozoite protein’s C-terminal domain
To improve malaria diagnostics novel detector systems with high specificity and sensitivity are required. The malarial antigen PfCSP-Cext was selected to generate the camelid single domain antibody sdAbCSP1, which is intended to be used as capture device for detecting PfCSP-Cext in serum of acutely ...
ORGANISM(S): Lama 
2024-08-28 | PXD051302 | Pride
Online nanolc esi-mse  item-two Offline nanoesi-mse 
UnknownAn Optimized Bayesian Hierarchical Two-Parameter Logistic Model for Small-Sample Item Calibration.
Not available
S-EPMC7262992 | biostudies-literature
UnknownMass Spectrometric ITEM-ONE and ITEM-TWO Analyses Confirm and Refine an Assembled Epitope of an Anti-Pertuzumab Affimer.
Not available
S-EPMC10813730 | biostudies-literature
UnknownControlling the Minimum Item Exposure Rate in Computerized Adaptive Testing: A Two-Stage Sympson-Hetter Procedure.
Not available
S-EPMC10664747 | biostudies-literature
UnknownValidity of a two-item screen for early psychosis.
Not available
S-EPMC6296822 | biostudies-literature
ProteomicsEpitope fine mapping by mass spectrometry: Investigations of immune complexes consisting of monoclonal anti-HpTGEKP antibody and zinc finger protein linker phospho-hexapeptides.
We describe the mass spectrometric analysis of the binding strength of a monoclonal mouse antibody (clone 18E9.D9, BioLegend, London, England) which was raised against the predominantly occuring phosphorylated linker sequence, HpTGEKP, of C2H2 zinc finger proteins (ZNF proteins) using a synthetic ...
ORGANISM(S): Homo sapiens (Human) 
2022-08-15 | PXD031150 | Pride
Item-two Off-line esi-ms Zinc finger-phosphopeptide Antiphosphopeptide-antibody Binding strength characterization 
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