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We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-23 | PXD016681 | Pride
Uropathogenic Escherichia coli (UPEC) are the most common cause of urinary tract infection (UTI). UPEC normally reside in the intestine, and during establishment of UTI, it undergoes metabolic adaptations, first to urine and then upon tissue invasion to the bladder cell interior. In order to underst...
ORGANISM(S): Escherichia coli 
2022-06-09 | PXD032049 | Pride
In the project « The inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation » Jianwen Zhou, Nikoline Lander Rasmussen, Vyacheslav Akimov, Blagoy Blagoev, Trond Lamark, Terje Johansen and Jörn Dengjel performed MS-based proteomics to study the f...
ORGANISM(S): Homo sapiens (Human) 
2022-08-07 | PXD027163 | Pride
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