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All yeast strains were in the genetic background W303-1A (MATa leu2-3,112 trp1-1 ura3-1 can1-100 ade2-1 his3-11,15). Gene expression profiles of the following mutant strains were compared with WT: YIA29 (mdl1::HIS3); YIA30 (yme1::KAN); YIA31 (mdl1::HIS3 yme1::KAN); 2 independent RNA preparations per...
ORGANISM(S): Saccharomyces cerevisiae 
To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(M-NM-^TsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gen...
ORGANISM(S): Bacillus thuringiensis 
A comparison of transcriptional profiles between MG Kan N15 (+) or MG Kan-Tos N15 (-), which are reference strains and MG Kan-Tos N15 (+) strain. The strain, MG Kan-Tos N15 (+), has linear genome which are made by MG Kan N15 (+) and MG Kan-Tos N15 (-).
ORGANISM(S): Escherichia coli 
Wildtype and ycgF::kan cells were grown in LB medium at 37°C to an OD(578 nm) of 0,7. Then the cultures were shifted to 16°C and blue-light irradiated for 3 hours.
ORGANISM(S): Escherichia coli 
Investigation of whole genome gene expression level changes in a Nitrosomonas europaea (ATCC 19718) wildtype and pFur::Kan mutant [kanamycin resistance cassette insertion in the promoter region of the fur gene (NE0616)] strains grown in Fe-replete and Fe-limited media. The Nitrosomonas europaea (ATC...
ORGANISM(S): Nitrosomonas europaea ATCC 19718 
A total of 25 modifications were mapped to 16S and 23S RNA in B. subtilis ribosome, however most of the genes responsible for these modification sites remain unknown. In this work, bottom-up oligonucleotide LC-MS/MS was used to detect rRNA modifications in five single gene knock-out strains: yydA, ...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2024-08-06 | PXD051518 | Pride
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