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Pseudomonas entomophila strain:23S Genome sequencing
Extremophilic archaea employ diverse chemical RNA modifications, providing a rich source of new enzymes for biotechnologically valuable RNA manipulations. Our understanding of the modified nucleoside profiles in Archaea, as well as the functions and dynamic regulation of specific RNA modifications i...
ORGANISM(S): Thermococcus kodakarensis 
2025-02-12 | PXD060644 | Pride
A total of 25 modifications were mapped to 16S and 23S RNA in B. subtilis ribosome, however most of the genes responsible for these modification sites remain unknown. In this work, bottom-up oligonucleotide LC-MS/MS was used to detect rRNA modifications in five single gene knock-out strains: yydA, ...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2024-08-06 | PXD051518 | Pride
OLA phytoplankton 23S
23S Metabarcoding of Titicaca Lake Raw sequence reads
Full-length 16S-23S rRNA gene sequences from pre and post PrEP specimens
Evaluating the Efficiency of RRN (16S-ITS-23S) Operon Sequencing
Post-transcriptional modifications are added to ribosomal RNAs (rRNAs) to govern ribosome biogenesis and to fine-tune protein biosynthesis. RlhA, a dedicated modification enzyme, adds a unique 5-hydroxycytidine (ho5C) at 23S rRNA position 2501 in E. coli and related bacteria. However, the molecular ...
ORGANISM(S): Escherichia coli 
2022-02-16 | PXD025875 | Pride
In this work, we show that 23S rRNA lacking the SRL can be efficiently reconstituted into 50S subunits, which exhibit full peptidyl transferase (PT) activity. Using cryo-EM and proteomic methods, we also further characterize affinity-purified SRL particles isolated from cells. Collectively, these da...
ORGANISM(S): Escherichia coli 
2025-05-07 | PXD056260 | Pride
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