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TranscriptomicsArabidopsis defense against Botrytis cinerea: chronology and regulation deciphered by high-resolution temporal transcriptomic analysis (time series)
A novel experimental design strategy (A Mead et al, in preparation), based on the principle of the M-bM-^@M-^\loop designM-bM-^@M-^], was developed to enable efficient extraction of information about key sample comparisons using a two-colour hybridisation experimental system. With 192 distinct sampl...
ORGANISM(S): Arabidopsis thaliana 
2012-10-02 | E-GEOD-29642 | biostudies-arrayexpress
TranscriptomicsContinuous T cell receptor signals maintain a functional regulatory T cell pool
During development, thymocytes bearing a moderately self-reactive T cell receptor (TCR) can be selected to become regulatory T (Treg) cells. Several observations suggest that also in the periphery mature Treg cells continuously receive self-reactive TCR signals. However, the importance of this inhe...
ORGANISM(S): Mus musculus 
2014-10-21 | E-GEOD-62532 | biostudies-arrayexpress
TranscriptomicsComparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain
In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in aerial mycelium of the tra1- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. TRA1-deleted mutant. 4 biological ...
ORGANISM(S): Magnaporthe oryzae 
2012-03-03 | E-GEOD-36224 | biostudies-arrayexpress
TranscriptomicsComparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain
In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in conidia of the tra1- mutant and the parental strain 70-15. Two-condition experiment, wild-type vs. mutant. 3 biological replicates
ORGANISM(S): Magnaporthe oryzae 
2012-03-03 | E-GEOD-36225 | biostudies-arrayexpress
TranscriptomicssiRNA of human MCF-7 cells
Overexpression of the AP-2γ transcription factor in breast tumours has been identified as an independent predictor of poor outcome and failure of hormone therapy, even in ER positive, ErbB2 negative tumours; markers of a more favourable prognosis. To understand further the role of AP-2γ in breast...
ORGANISM(S): Homo sapiens 
2009-12-19 | E-GEOD-15481 | biostudies-arrayexpress
TranscriptomicsThe role of the Tra1p transcription factor of Magnaporthe oryzae in spore adhesion and pathogenic development
This SuperSeries is composed of the following subset Series: GSE36224: Comparison of transcript abundance in aerial mycelium of the Magnaporthe oryzae TRA1-deleted mutant and its parental strain GSE36225: Comparison of transcript abundance in ungerminated spores of the Magnaporthe oryzae TRA1-delete...
ORGANISM(S): Magnaporthe oryzae 
2012-03-03 | E-GEOD-36226 | biostudies-arrayexpress
TranscriptomicsWhole-transcriptome, high-throughput RNA sequence analysis of the bovine macrophage response to MycobacteriumM-BM- bovis infection in vitro
Background: MycobacteriumM-BM- bovis, the causative agent of bovine tuberculosis, is an intracellular pathogen that can persist inside host macrophages during infection via a diverse range of mechanisms that subvert the host immune response. In the current study, we have analysed and compared the tr...
ORGANISM(S): Bos taurus 
2013-03-26 | E-GEOD-45439 | biostudies-arrayexpress
GenomicsWGSeq_HealthyHuman_DATA_SET_xmas_MB_2013_07_10 - samples
HSC73_clone: Bone marrow mononuclear cells from the healthy 73 years old female were thawed and labeled with Alexa-Fluor 488-conjugated anti-CD34 (581, Biolegend), Alexa-Fluor 700-conjugated anti-CD38 (HIT2, eBioscience), a cocktail of APC-conjugated lineage antibodies consisting of anti-CD4 (RPA-T4...
EGAD00001000666 | EGA
TranscriptomicsTranscription profiling of human monocytic cell line, THP-1 cells were stimulated 4 hours with chemokine MIG or CCL2/MCP-1
The objective of the study was to discover new functions of chemokine CXCL9/MIG, a cationic chemokine that has antimicrobial properties. Human monocytic cell line, THP-1 cells were stimulated 4 hours with chemokine MIG or CCL2/MCP-1 (a chemokine that is not positively charged and has no antimicrobi...
ORGANISM(S): Homo sapiens 
2010-07-01 | E-FPMI-5 | biostudies-arrayexpress
TranscriptomicsFluorochrome-based definition of naturally occurring Foxp3+ regulatory T cells of intra- and extrathymic origin
Here, we report on experiments in double-transgenic mice, in which RFP is expressed in all Foxp3+ Treg cells, whereas Foxp3-dependent GFP expression is exclusively confined to intrathymically induced Foxp3+ Treg cells. This novel molecular genetic tool enabled us to faithfully track and characterize...
ORGANISM(S): Mus musculus 
2014-07-09 | E-GEOD-59202 | biostudies-arrayexpress
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