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Fungal resistant and susceptible maize genotypes were subjected to Aspergillus flavus spore inoculation and kernels around the infected area were collected 4 days after inoculation. Uninoculated kernels were also collected at 4 days. Microarray experiment was performed to compare the transcriptional...
ORGANISM(S): Zea mays 
gp130Act cDNA was PCR-amplified and inserted into a plasmid containing the 12.4-kb Villin promoter. The 15.7-kb expression cassette was excised by PmeI digestion, purified, and injected into fertilized C57BL/6 oocytes to obtain founder mice, two of which transmitted the gp130Act transgene. Small int...
ORGANISM(S): Mus musculus 
Pancreas and spleen were microdissected from wild type (C57B6/SV129 background) E14.5 embryos and P0 newborn pups. Pools of 30-60 tissues were used. Tissues were digested for 1 hour at 37°C in HBSS/0.1% collagenase A/20 µg/ml DNase I, followed by dissociation of the cell clusters into single cells...
ORGANISM(S): Mus musculus 
Total RNA was isolated from SUM-44 cells subjected to 5 days of puromycin selection following infection with lentiviral shRNA constructs against NSD3-S or ESR1 as well as LacZ control. Samples were harvested in triplicate and prepared and sequenced simultaneously.
ORGANISM(S): Homo sapiens 
Hornyhead turbot (Pleuronichthys verticalis) captured near sewage outfalls are used as sentinel fish for monitoring exposure to industrial and agricultural chemicals of ~20 million people living in coastal southern California. Although analyses of hormones in blood and organ morphology and histology...
ORGANISM(S): Pleuronichthys verticalis 
Laboratory tests with marine flatfish were conducted to investigate associations among gene expression, higher biological responses and wastewater effluent exposure. Previous studies showed molecular responses such as elevated concentrations of plasma estradiol and vitellogenin in wild male hornyhe...
ORGANISM(S): Pleuronichthys verticalis 
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