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ProteomicsLimits for resolving isobaric tandem mass tag reporter ions using phase constrained spectrum deconvolution
A popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT) which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-05 | PXD011009 | Pride
Q exactive hf-x Phase constrained spectrum deconvolution Tmt Resolution Quantitative proteomics 
OtherRapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer.
Data from ProteomeXchange, PXD ID: PXD001305. Experiment: UPLC9_RJC_DEV_HeLa_03, file: 20140418_QE7_UPLC9_RJC_DEV_HeLaFrac10_03.mzml. Published as part of J Proteome Res. 2014 Oct 28 . From the Abstract: {{i}} Here, we investigate faster sequencing speed of the latest Q Exactive HF mass spectromete...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM11210030961 | GPMDB
Resource Reanalysis 
OtherRapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer.
Data from ProteomeXchange, PXD ID: PXD001305. Experiment: UPLC9_RJC_DEV_HeLa_02, file: 20140418_QE7_UPLC9_RJC_DEV_HeLaFrac1_02.mzml. Published as part of J Proteome Res. 2014 Oct 28 . From the Abstract: {{i}} Here, we investigate faster sequencing speed of the latest Q Exactive HF mass spectrometer...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320004807 | GPMDB
Resource Reanalysis 
ProteomicsAnalytical framework for peptidomics applied to large scale neuropeptide identification
Endogenous peptides are extracted and seperated with nLC with HCD fragmentation on a LTQ Orbitrap Velos. Data analysis was done with Mascot and MaxQuant
ORGANISM(S): Rattus Rattus (ncbitaxon:10117) 
2017-02-16 | MSV000080552 | MassIVE
ProteomicsCombining mass spectrometry and machine learning to discover bioactive peptides
Mammalien peptidome map using seven metabolic active tissues and four different strains. 150.000+ identified peptides. Machine learning applied to score peptides based on similarity to known peptides. Functional validation of high scoring peptides.
ORGANISM(S): Mus musculus (Mouse) 
2022-10-20 | PXD022225 | Pride
Peptidomics Tissues Mouse Bioactive peptides Peptidome 
ProteomicsRapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer
The faster sequencing speed available on the latest Q Exactive HF mass spectrometer is investigated and evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments. Also the instrument capabilities for offline high pH reversed-phase peptide frac...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-01 | MSV000079548 | MassIVE
ProteomicsHeLa proteome of 12,250 protein-coding genes
We developed an optimized multi-shot proteomics workflow based on high-resolution offline high pH reversed-phase peptide separation of high peptide loads collecting many fractions that were in turn analyzed by short online chromatographic separations and fast peptide sequencing using orbitrap tandem...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000081563 | MassIVE
ProteomicsHuman dental calculus LC-MS/MS
The purpose of this study was to investigate oral microbiome and host proteins in archaeological human dental tissues using a shotgun proteomics approach. The research focuses on dental calculus (mineralized plaque), dentine, a carious lesion, and an alveolar bone abscess from the medieval site of D...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606) Ovis (ncbitaxon:9935) 
2016-10-23 | MSV000080279 | MassIVE
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