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GenomicsHigh salt reduces the activation of IL-4+IL-13 stimulated macrophages
High salt (NaCl) intake is a major risk factor for cardiovascular disease; high NaCl has also been shown to be a powerful modulator of pro-inflammatory immune cell polarization. To investigate the effect of high NaCl on the polarization of M(IL-4+IL-13) macrophages we performed gene expression analy...
ORGANISM(S): Mus musculus 
2015-02-07 | E-MTAB-3140 | biostudies-arrayexpress
TranscriptomicsClariomS Array to determine the impact of whole body adenylyl cyclase 5 (Adcy5) knockout on male/female epigonadal white adipos tissue (eWAT) gene expression
We analyzed the effect of whole body Adcy5 knockout on adipose tissue gene expression profiles. Therefore, we isolated RNA, examined RNA integrity and concentration on an Agilent Fragment Analyzer using the HS RNA Kit. cRNA was prepared from 100 ng of total RNA hybridized to GeneChip Clariom S array...
ORGANISM(S): Mus musculus 
2021-04-19 | E-MTAB-9417 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing and that disr...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-13594 | biostudies-arrayexpress
GenomicsSalt augments macrophage-driven host defense in the skin
Immune cells regulate a hypertonic microenvironment in the skin; however, possible functions of increased skin Na+ concentrations are unknown. We found that Na+ accumulated at the site of bacterial skin infections in humans and in mice. We used the protozoan parasite Leishmania (L.) major as a model...
ORGANISM(S): Mus musculus 
2015-02-26 | E-MTAB-3343 | biostudies-arrayexpress
ProteomicsHigh-throughput phosphoproteomics reveals in vivo insulin signaling dynamics
In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and p...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-06-25 | MSV000082502 | MassIVE
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model, AVV9-ACADVL rescue
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing, and that dis...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-14396 | biostudies-arrayexpress
TranscriptomicsHeart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model, nuclear sequencing without treatment
Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing, and that dis...
ORGANISM(S): Mus musculus 
2026-05-15 | E-MTAB-14401 | biostudies-arrayexpress
TranscriptomicsA Myc-dependent role for cohesin in the heterokaryon-mediated reprogramming of somatic nuclei
Analysis of gene expression changes in mESCs where the cohesin subunit Scc1 has been depleted. There are 6 samples. 3 Scc1 wt/wt ERT2-Cre biological replicas and 3 Scc1 lox/lox ERT2-Cre biological replicas, all treated with 4'-OHT 80nM final concentration for 24h. Scc1 wt/wt and Scc1 lox/lox ERT2-Cr...
ORGANISM(S): Mus musculus 
2015-02-19 | E-GEOD-62325 | biostudies-arrayexpress
GenomicsMicroRNAs maintain bivalency in mouse embryonic stem cells [ChIP-seq]
The protein Dicer is required for microRNA (miRNA) biogenesis, and therefore Dicer-deficient cells lack all mature, functional miRNAs. Here we investigated the binding of the PRC2 component Ezh2 in wildtype and Dicer-deficient mouse embryonic stem cells genomewide using ChIP-sequencing analysis. Exa...
ORGANISM(S): Mus musculus 
2016-06-02 | E-GEOD-60160 | biostudies-arrayexpress
TranscriptomicsNuclear SLIC-CAGE of human cortical neurons from Cornelia de Lange Syndrome patients and control individuals
We used SLIC-CAGE to map transcriptional start sites in cortical neurons from Cornelia de Lange Syndrome (CdLS) patients and control individuals. SLIC-CAGE was performed using nuclear RNA isolated from pre-frontal cortical grey matter. Usage of nuclear RNA allows enrichment of unstable RNAs, such as...
ORGANISM(S): Homo sapiens 
2021-02-27 | E-MTAB-9045 | biostudies-arrayexpress
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