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ProteomicsKinome analysis of CD151 overexpressing Jurkat T cells
The effect of CD151 expression onto the kinome of Jurkat T cells was assessed using kinome analysis. CD151 was expressed in Jurkat T cells by retroviral transduction based on a pMSCV vector. Entrez Gene: 977 UniProtKB: P48509 Jurkat T cells were transduced with the MSCV-CD151 vector and successfull...
ORGANISM(S): Homo sapiens 
2017-09-30 | E-MTAB-5978 | biostudies-arrayexpress
ProteomicsIdentification and functional annotation of the Src-regulated kinome, part 2
Despite significant progress, our understanding of how specific oncogenes transform cells is still limited and likely underestimates the complexity of downstream signalling events. Herein, we describe a novel, integrated approach that addresses this knowledge gap. This utilizes mass spectrometry-bas...
ORGANISM(S): Homo sapiens (Human) 
2018-11-21 | PXD011146 | Pride
Lc-msms Kinome Phosphoproteome 
TranscriptomicsshRNA cassette sequencing from disomic and trisomic fibroblasts cultured in the presence of shRNA kinome library for 14 days
Kinome-wide shRNA screen to identify kinases differentially required for survival of cells disomic and trisomic for chromosome 21 Cells were transduced in triplicate and propagated for 14 days to allow for enrichement or depletion of shRNAs from the population
ORGANISM(S): Homo sapiens 
2016-07-29 | E-GEOD-79840 | biostudies-arrayexpress
ProteomicsProbing the global kinome and phosphoproteome in Chlamydomonas reinhardtii via sequential enrichment and quantitative proteomics
Identifying dynamic protein phosphorylation events is critical for understanding kinase/phosphatase regulated signaling pathways. To date, protein phosphorylation and kinase expression have been examined independently in photosynthetic organisms. Presented here is a method to study the global kinome...
ORGANISM(S): Chlamydomonas reinhardtii 
2016-10-05 | PXD004681 | Pride
Phosphoproteomics Quantitative label-free proteomics Kinome Mass spectrometry Chlamydomonas reinhardtii 
ProteomicsMYC-dependent kinome in pancreatic cancer
We recently established the ERK-dependent transcriptome and phosphoproteome in KRAS-mutant pancreatic ductal adenocarcinoma (PDAC). Of 1,718 ERK-dependent genes, 143 encode transcription factors. Of 2,123 ERK-dependent phosphoproteins, 98 are transcription factors. Despite this complexity, we observ...
ORGANISM(S): Homo sapiens (Human) 
2026-01-15 | PXD061413 | Pride
Mib-ms Myc Pancreatic cancer Kinome 
ProteomicsCharacterization of the Src-regulated kinome identifies SGK1 as a key mediator of Src-induced transformation
Despite significant progress, our understanding of how specific oncogenes transform cells is still limited and likely underestimates the complexity of downstream signalling events. Herein, we describe a novel, integrated approach that addresses this knowledge gap. This utilizes mass spectrometry-bas...
ORGANISM(S): Homo sapiens (Human) 
2019-01-14 | PXD010687 | Pride
Breast cancer Cancer Src Kinome 
ProteomicsDetermining kinome changes in response to Cytomegalovirus US28 expression and identifying US28 interacting proteins
Cytomegalovirus (CMV) reactivation of latent infection following immune dysregulation remains a serious risk for patients, often causing significant morbidity and mortality. Herein, we demonstrate the CMV-encoded GPCR, US28, in coordination with cellular Ephrin receptor A2 (EphA2), attenuates MAPK s...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD033391 | Pride
Viral infection Affinity purification-mass spectrometry Kinome 
GenomicsIntrinsic Resistance to MEK Inhibition Through BET Protein Mediated Kinome
Intrinsic Resistance to MEK Inhibition Through BET Protein Mediated Kinome
PRJNA525696 | ENA
GenomicsTumor-intrinsic kinome landscape of pancreatic cancer reveals new therapeutic approaches
Tumor-intrinsic kinome landscape of pancreatic cancer reveals new therapeutic approaches
PRJNA1168601 | ENA
GenomicsDefining the Triple Negative Breast Cancer Kinome Response to GSK1120212
Defining the Triple Negative Breast Cancer Kinome Response to GSK1120212
PRJNA393488 | ENA
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