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TranscriptomicsRNA-seq profiling of microdissected rat renal tubule segments
We sequenced mRNAs from glomeruli and 14 different rat renal tubule segments collected by hand microdissection. Collagenase-digested rat renal tubule segments were collected by hand microdissection. Poly(A)-mRNAs were captured from cell lysate and sequenced using paired-end protocol.
ORGANISM(S): Rattus norvegicus 
2015-03-31 | E-GEOD-56743 | biostudies-arrayexpress
ProteomicsBayesian Analysis of Dynamic Phosphoproteomic Data Identifies Protein Kinases Mediating Responses to a Galphas-Coupled GPCR
A major goal in the discovery of signaling networks is to identify regulated phosphorylation sites and map them to the protein kinases responsible for their phosphorylation. The V2 vasopressin receptor is a Galphas-coupled GPCR that is responsible for regulation of renal water excretion through cont...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-06-17 | PXD031332 | Pride
Phospho proteomics Vasopression Protein kinases Baysian analysis Gpcr 
ProteomicsSignaling Pathways in Vasopressin-Mediated Regulation of Aquaporin-2
Vasopressin controls water excretion by regulating the water channel protein, aquaporin 2. Vasopressin acts in the renal collecting duct by binding to a G-protein coupled receptor and activating protein kinase A (PKA). Signaling events beyond PKA are largely unknown. Ultra-deep phosphoproteomic anal...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-05-25 | PXD011245 | Pride
Collecting duct Lc-ms/ms Phosphorylation 
ProteomicsSerine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct
Phosphorylation of the aquaporin-2 (AQP2) water channel at four COOH-terminal serines plays a central role in the regulation of water permeability of the renal collecting duct. The level of phosphorylation at these sites is determined by a balance between phosphorylation by protein kinases and depho...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-10-26 | PXD005488 | Pride
Systems biology  collecting duct  kidney  vasopressin  lc-ms/ms 
ProteomicsPKA-Independent Vasopressin Signaling in Renal Collecting Duct
Vasopressin regulates renal water excretion by binding to the Gs-coupled vasopressin receptor (V2R) in collecting duct cells, resulting in cyclic AMP-dependent increases in epithelial water permeability through regulation of the aquaporin-2 (AQP2) water channel. Our prior studies showed that CRISPR...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-05 | PXD015719 | Pride
Mpkccd Phosphoproteomics Ampk Silac Prm-ms 
ProteomicsCRISPR-Cas9/phosphoproteomics identifies Myosin Light Chain Kinase dependent signaling in kidney epithelial cells
Prior studies have implicated myosin light chain kinase (MLCK) in the regulation of aqua-porin-2 (AQP2) in the renal collecting duct. To discover signaling targets of MLCK, we deleted the Mylk- gene to obtain MLCK-null mpkCCD cells and carried out comprehensive phosphopro-teomics using the SILAC met...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-04 | PXD014985 | Pride
Nuclear membrane Mass spectrometry Vasopressin Aqp2 Endosomal trafficking Sted microscopy 
ProteomicsIdentification of β-Catenin-Interacting Proteins in Nuclei of Aquaporin-2-Expressing Rat Collecting Duct Cells
The gene encoding the aquaporin-2 water channel is regulated transcriptionally in response to vasopressin. In the renal collecting duct, vasopressin stimulates the nuclear translocation and phosphorylation (at Ser552) of β-catenin, a multifunctional protein that can act as a transcriptional co-regul...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-10-26 | PXD005792 | Pride
Transcription Transcriptional co-regulator Aquaporin-2 Kidney 
ProteomicsH89 Phosphoproteomics in PKA-Null Cells
Protein phosphorylation plays a crucial role in propagating cellular responses to both internal and external cues. One of the most important kinases responsible for protein phosphorylation is protein kinase A (PKA). N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulphonamide (H89) is often used as a...
ORGANISM(S): Mus musculus (Mouse) 
2019-03-28 | PXD011167 | Pride
Kinase inhibitor H89 Pka Phosphoproteomics 
ProteomicsProteomic analysis of microdissected renal tubules
Kidney is a vital organ which help maintain body homeostasis. Three related processes including glomerular filtration, tubular reabsorption and secretion are precisely regulated in the kidney in response to changes in the amount of both water and various solutes. To understand how the kidney can ach...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-04-20 | PXD016958 | Pride
Microdissection Physiology Renal tubule Kidney Lc-ms/ms 
ProteomicsCircadian Gene Expression in Mouse Renal Proximal Tubule
Circadian variability in kidney function has long been recognized but is often ignored as a potential confounding variable in in vivo physiological experiments. To provide a guide for physiological studies on the kidney proximal tubule, we have now created a data resource consisting of expression le...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-10 | PXD039006 | Pride
Circadian variation Proteomics Proximal tubule Transcriptomics 
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