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ProteomicsMALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening
To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze / thaw cycles and to reduce high costs for shipping and storage, we sought to dev...
ORGANISM(S): Homo sapiens (Human) 
2021-05-20 | PXD024669 | Pride
Intact serum protein analysis Maldi-ms profiling Fast frozen serum Serum proteolysis analysis Dried serum spots 
ProteomicsCharacterization of the Zein Protein Composition in Maize Flour Extracts
Highly homogenous zein protein was isolated from maize kernels in an environment-friendly process using 95 % ethanol as solvent. High purity of the zein protein product was determined by SDS PAGE analysis and by 2 D gel electrophoresis followed by MALDI-ToF-MS peptide mass fingerprinting after in-ge...
ORGANISM(S): Zea mays (Maize) 
2019-06-11 | PXD012849 | Pride
Zein proteins Peptide mass fingerprinting Data base search Maize Maldi-ms Custom-built sequence data base Flour 2d gel electrophoresis 
ProteomicsThe mass spectrometric intact transition epitope mapping method supports protein engineering of foldon trimer variants
The wild-type foldon (foldon 0) is the C-terminal trimerization domain of the bacteriophage T4 Fibritin foldon (T4Ff). T4Ff’s amino acid sequence is GYIPEAPRDGQAYVRKDGEWVLLSTFL (27 amino acid residues; single letter code). Foldons 0, 1-6 were synthesized by solid phase peptide synthesis and in foldo...
ORGANISM(S): Enterobacteria phage T4 (Bacteriophage T4) 
2025-11-28 | PXD064233 | Pride
ProteomicsIntact Transition Epitope Mapping – Serological Inspection by Epitope EXtraction (ITEM – SIX)
Precision medicine requests accurate serological inspections to precisely stratify patients for targeted treatment. Intact transition epitope mapping analysis proved seroconversion of a model organism's serum when spiked with a monoclonal murine anti-ovalbumin antibody with epitope-resolution. Isola...
ORGANISM(S): Gallus gallus (Chicken) 
2023-03-31 | PXD040754 | Pride
Epitope mapping Item-six Blood serum Seroconversion Offline nanoesi-ms Immune complex analysis 
ProteomicsCharacterization of Phosphorylation-dependent Antibody Binding to Cancer-mutated Linkers of C2H2 Zinc Finger Proteins by Intact Transition Epitope Mapping - Thermodynamic Weak-force Order Analysis.
We describe mass spectrometric antibody-peptide binding motif analyses of a monoclonal antibody (clone 18E9.D9, BioLegend, London, England) from mouse which was raised against the predominantly occuring phosphorylated linker sequence HpTGEKP of human C2H2 zinc finger proteins. As the anti-HpTGEKP an...
ORGANISM(S): Homo sapiens (Human) 
2023-01-25 | PXD035818 | Pride
Item-two Off-line esi-ms Uterine corpus endometrial carcinoma Skin cutaneous melanoma Zinc finger-phosphopeptide Antiphosphopeptide-antibody Colon and rectal adenocarcinomas 
ProteomicsMass spectrometric ITEM-TWO analyses of interactions between human cardiac Troponin T (hcTnT) peptides and the M7 or the M11.7 antiTnT antibodies.
Molecular characterization of antibodies M7, and M11.7, as well as the antiTNF alpha antibody in addition to the synthetic peptides P11-P17 and P21-P32. The M7 and M11.7 anti-human cardiac Troponin T (antihcTnT) antibodies from Roche Diagnostics Deutschland GmbH (Mannheim, Germany) and the antiTNF a...
ORGANISM(S): Homo sapiens (Human) 
2025-05-20 | PXD058812 | Pride
Item-two Troponin t Offline nanoesi-ms 
ProteomicsIntact Transition Epitope Mapping - Forces of Interactions between Variants of Equivalent macromolecules (ITEM-FIVE)
Foldon (F) is a specific partial sequence from the C-terminal domain of T4 fibritin, which is found at the neck of the T4 bacteriophage. The amino acid sequence (27 residues) in single letter code is GYIPEAPRDGQAYVRKDGEWVLLSTFL. In neutral solution (pH 6.9) it forms a homo-trimer (F-F-F). Biotinylat...
ORGANISM(S): Enterobacteria phage T4 (Bacteriophage T4) 
2024-04-29 | PXD044721 | Pride
Item-two Hetero-trimer Offline esi-ms Foldon Complex dissociation Homo-trimer Gas phase 
ProteomicsMass spectrometry-guided molecular modeling predicts the interaction interface for a camelid single-domain antibody targeting the Plasmodium falciparum circumsporozoite protein’s C-terminal domain
To improve malaria diagnostics novel detector systems with high specificity and sensitivity are required. The malarial antigen PfCSP-Cext was selected to generate the camelid single domain antibody sdAbCSP1, which is intended to be used as capture device for detecting PfCSP-Cext in serum of acutely ...
ORGANISM(S): Lama 
2024-08-28 | PXD051302 | Pride
Online nanolc esi-mse  item-two Offline nanoesi-mse 
ProteomicsMass spectrometric ITEM-ONE analysis confirms and refines an assembled affimer binding site on the HER-2 targeting therapeutic antibody Pertuzumab
The Pertuzumab-binding affimer 00557_709097 was incubated either with peptides from tryptically digested Pertuzumab or with selected synthesized peptides. Affimer-peptide complexes were subjected to nano electrospray mass spectrometric ITEM analyses in order to identify the affimer’s epitope on Pert...
ORGANISM(S): Homo sapiens (Human) 
2024-04-09 | PXD043203 | Pride
Pertuzumab Item Affimer Offline nanoesi-ms 
ProteomicsConformational changes of shapeshifting protein G’e and immunoglobulin IgG probed by nanoESI ion mobility mass spectrometry.
Integrative structural biology approaches make use of artificial intelligence-assisted modelling on one side and of experimental analyses on the other for in-depth characterization of higher order protein structures as well as physiological structural changes. Calculations of CCS values are based on...
ORGANISM(S): Bos taurus (Bovine) Streptococcus sp. 'group G' Homo sapiens (Human) Gallus gallus (Chicken) Apis mellifera (Honeybee) Sus scrofa domesticus (domestic pig) 
2026-09-01 | PXD076256 | Pride
Offline esi-ms Ims-ms 
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