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ProteomicsFollicular cells Top-down proteomic
In mammals, the capacity of the female germ cell, the oocyte, to develop into embryo is acquired throughout meiotic maturation. Immature oocyte cannot be fertilized while mature oocyte is apt to accept spermatozoa and to develop an embryo. In a follicle, the oocyte is surrounded by mural granulosa c...
ORGANISM(S): Bos taurus (Bovine) 
2017-04-10 | PXD004892 | Pride
Oocytes Μlc-ms/ms Cumulus cells Top-down proteomic Granulosa cells 
ProteomicsChicken spermatozoa protein extract analyzed by Top-down HRMS
Currently, evaluation of quality semen is based on traditional in vitro sperm tests as measures of motility, viability and acrosomic reaction. However, the results are not always well correlated with real male fertility. In the present study, we evaluated Intact Cell MALDI-TOF Mass Spectrometry (ICM...
ORGANISM(S): Gallus gallus (Chicken) 
2016-04-11 | PXD002768 | Pride
Chicken Top-down Spermatozoa 
ProteomicsAnalysis of epididymal sperm maturation by top-down mass spectrometry
Spermatozoa Top-down analyses of different epididymal regions to identify endogenous biomolecules.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2014-12-16 | PXD001303 | Pride
Boar Epididymal spermatozoa Top-down 
UnknownData in support of peptidomic analysis of spermatozoa during epididymal maturation.
Not available
S-EPMC4459559 | biostudies-literature
UnknownData on endogenous bovine ovarian follicular cells peptides and small proteins obtained through Top-down High Resolution Mass Spectrometry.
Not available
S-EPMC5454127 | biostudies-literature
ProteomicsQualitative and quantitative analysis of chicken semen
Chicken proteome of ejaculated spermatozoa (SPZ) and seminal plasma (SP) and evaluation of the molecular phenotype related to sperm quality were investigated using GeLC-MS/MS approach.
ORGANISM(S): Gallus gallus (Chicken) 
2014-08-22 | PXD001254 | Pride
Chicken Seminal plasma Proteome Spermatozoa Label free 
ProteomicsComparative analysis of chicken seminal plasma
Semen from 11 chickens was collected, supplemented with anti-proteases and centrifuged to separate sperm cells and fluids. 25µg of proteins for each chicken were included in-gel without fractionnation (1 band) followed by Coomassie Blue staining. After reduction and alkylation, proteins were in-gel ...
ORGANISM(S): Gallus gallus (Chicken) 
2014-08-06 | PXD000287 | Pride
Lc-msms Chicken Seminal plasma 
UnknownData for chicken semen proteome and label free quantitative analyses displaying sperm quality biomarkers.
Not available
S-EPMC4459768 | biostudies-literature
ProteomicsUterine fluid proteomic analysis of fertile and subfertile hens before and after insemination
Avian uterine fluid (UF) and uterovaginal sperm storage tubules (SST) are key components for acceptance of sperm in SST, sperm functions maintenance during weeks, sperm release out of SST, and their ascending through the uterus. The study of UF and SST is essential to better understand sperm storage...
ORGANISM(S): Gallus gallus (Chicken) 
2019-08-29 | PXD013514 | Pride
Insemination Sperm storage Uterin fluid 
ProteomicsProteomic changes associated with sperm fertilizing ability in meat type roosters
The molecular basis of male infertility is still not well understood. Furthermore, in vitro sperm quality tests are poorly correlated to in vivo fertility. In order to better understand sperm biology and determine potential fertility biomarkers, we used a quantitative bottom-up proteomic approach to...
ORGANISM(S): Gallus gallus (Chicken) 
2021-08-17 | PXD022322 | Pride
Fertility Chicken Spermatozoa 
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