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ProteomicsComparative analysis of chicken seminal plasma
Semen from 11 chickens was collected, supplemented with anti-proteases and centrifuged to separate sperm cells and fluids. 25µg of proteins for each chicken were included in-gel without fractionnation (1 band) followed by Coomassie Blue staining. After reduction and alkylation, proteins were in-gel ...
ORGANISM(S): Gallus gallus (Chicken) 
2014-08-06 | PXD000287 | Pride
Lc-msms Chicken Seminal plasma 
ProteomicsFollicular cells Top-down proteomic
In mammals, the capacity of the female germ cell, the oocyte, to develop into embryo is acquired throughout meiotic maturation. Immature oocyte cannot be fertilized while mature oocyte is apt to accept spermatozoa and to develop an embryo. In a follicle, the oocyte is surrounded by mural granulosa c...
ORGANISM(S): Bos taurus (Bovine) 
2017-04-10 | PXD004892 | Pride
Oocytes Μlc-ms/ms Cumulus cells Top-down proteomic Granulosa cells 
ProteomicsAnalysis of epididymal sperm maturation by top-down mass spectrometry
Spermatozoa Top-down analyses of different epididymal regions to identify endogenous biomolecules.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2014-12-16 | PXD001303 | Pride
Boar Epididymal spermatozoa Top-down 
ProteomicsQualitative and quantitative analysis of chicken semen
Chicken proteome of ejaculated spermatozoa (SPZ) and seminal plasma (SP) and evaluation of the molecular phenotype related to sperm quality were investigated using GeLC-MS/MS approach.
ORGANISM(S): Gallus gallus (Chicken) 
2014-08-22 | PXD001254 | Pride
Chicken Seminal plasma Proteome Spermatozoa Label free 
ProteomicsChicken spermatozoa protein extract analyzed by Top-down HRMS
Currently, evaluation of quality semen is based on traditional in vitro sperm tests as measures of motility, viability and acrosomic reaction. However, the results are not always well correlated with real male fertility. In the present study, we evaluated Intact Cell MALDI-TOF Mass Spectrometry (ICM...
ORGANISM(S): Gallus gallus (Chicken) 
2016-04-11 | PXD002768 | Pride
Chicken Top-down Spermatozoa 
ProteomicsProteomic changes associated with sperm fertilizing ability in meat type roosters
The molecular basis of male infertility is still not well understood. Furthermore, in vitro sperm quality tests are poorly correlated to in vivo fertility. In order to better understand sperm biology and determine potential fertility biomarkers, we used a quantitative bottom-up proteomic approach to...
ORGANISM(S): Gallus gallus (Chicken) 
2021-08-17 | PXD022322 | Pride
Fertility Chicken Spermatozoa 
ProteomicsOral exposure to bisphenol S associated with alterations in the proteome of granulosa cells in ewes with contrasted metabolic status.
Bisphenol S (BPS) is a substitute for bisphenol A in plastic manufacturing, the BPA being an endocrine disruptor that alters granulosa cell (GC) functions. Furthermore, bisphenols are able to modulate lipid metabolism and adipogenesis. The aim of this study was therefore to characterise the effects ...
ORGANISM(S): Ovis aries 
2024-11-07 | PXD047107 | Pride
Ovine Granulosa cells Bisphenol s Gelc-ms/ms Ovary 
ProteomicsGeLC-MS/MS analysis of hen egg vitelline membrane from freshly-laid eggs and eggs stored for 28 days at 4°C and 20°C
The hen egg vitelline membrane is a protein layer separating the yolk content from the egg white. The integrity of egg vitelline membrane is important for both the shell egg and the egg product industries. However, its physical properties are altered during prolonged storage, depending on storage te...
ORGANISM(S): Gallus gallus (Chicken) 
2026-03-16 | PXD066675 | Pride
Sds-page Egg Vitelline membrane Chicken Nanolc-ms/ms Storage 
ProteomicsOral exposure to bisphenol S is associated with alterations in oviduct proteome with aggravated effects in overweight females in an ovine model
In mammals, the final maturation and transport of gametes, fertilization and early embryo development take place in the oviducts. Bisphenol S (BPS) is a substitute for bisphenol A in plastic manufacturing and, as a potential endocrine disruptor, may alter the oviduct physiology. Furthermore, bisphen...
ORGANISM(S): Ovis aries 
2024-05-28 | PXD044858 | Pride
Ovine Oviduct Bisphenol s Fluid Gelc-ms/ms 
ProteomicsIdentification of differentially expressed proteins in human pluripotent stem cell derived into dopaminergic neurons
Dopaminergic neurons located in the ventral midbrain can be broadly subdivided into two distinct subpopulations. Substantia nigra (SN) dopaminergic neurons are highly sensitive to toxic insults and selectively degenerate in Parkinson’s disease, while ventral tegmental area (VTA) dopaminergic neurons...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD028639 | Pride
Pluripotent stem cells Dopaninergic neurons Parkinson disease 
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