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MetabolomicsLC-MS/MS Quantification of Nuclear α-KG in NSCLC
This study contains targeted LC-MS/MS quantification of α-ketoglutarate (α-KG) in NSCLC cells using 13C-labeled α-KG internal standard. Nuclear α-KG levels were measured in A549, A549/DTX, PC9, and PC9/OR cells. In addition, nuclear α-KG was quantified in GLUD1-overexpressing A549 cells and GLUD1-kn...
2026-07-21 | MTBLS15115 | MetaboLights
MetabolomicsAdvancing Reef Monitoring Techniques through Exometabolomics: Quantification of Labile Dissolved Organic Metabolites on Coral Reefs

Extracellular chemical cues constitute much of the language of life among marine organisms from microbes to mammals. These chemical cues shape foraging and feeding strategies, symbiotic interactions, selection of mates and habitats, and the transfer of energy and nutrient...

2025-07-16 | MTBLS9008 | MetaboLights
MetabolomicsA combined direct infusion/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with 13C- internal standards
We propose a fully automated novel workflow for lipidomics based on flow injection, followed by liquid chromatography-high-resolution mass spectrometry (FI/LC-HRMS). The workflow combined in-depth characterization of the lipidome achieved via reversed-phase LC-HRMS with absolute quantification by us...
2021-07-01 | MTBLS1876 | MetaboLights
MetabolomicsStable isotope-resolved metabolomics of WT, GYG1 KO, and GYG2 KO human embryonic stem cells using 13C-labeled glucose and palmitate
Proper regulation of glycogen metabolism is fundamental to cellular energy homeostasis, and its disruption is associated with various metabolic disorders, including glycogen storage diseases (GSDs) and potentially diabetes. Despite glycogen’s role as an essential energy reservoir, the mechanisms gov...
2025-07-15 | MTBLS12586 | MetaboLights
ProteomicsRelative quantification of 15N labeled acinus-2 pinin-1 and 14N labeled Col through Data Dependent Acquisition to obtain peptide information for targeted Parallel Reaction Monitoring experiment
To examine the properties of 14N and 15N labeled peptides using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer we first set up this DDA experiment. The purpose of this experiment was to choose peptides that are good candidates for targeted quantification. Th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD030098 | Pride
Arabidopsis Quantification Data dependent acquisition 15n metabolic labeling Relative quantification Silac Protein prospector 
TranscriptomicsAbsolute quantification of miRNAs
MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting tempera...
ORGANISM(S): Mus musculus 
2010-06-20 | E-GEOD-15835 | biostudies-arrayexpress
TranscriptomicsRelative quantification of miRNAs across multiple experiments
MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting tempera...
ORGANISM(S): synthetic construct 
2010-06-20 | E-GEOD-15833 | biostudies-arrayexpress
TranscriptomicsAbsolute quantification of CD34(+)/CD133(-) hematopoietic stem cells
MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting tempera...
ORGANISM(S): synthetic construct 
2009-10-26 | E-GEOD-15831 | biostudies-arrayexpress
GenomicsLong-TUC-seq is a robust method for quantification of metabolically labeled full-length isoforms
Long-TUC-seq is a robust method for quantification of metabolically labeled full-length isoforms
PRJNA629096 | ENA
ProteomicsUsing targeted quantification (PRM) to quantify proteins between wild type Col and acinus-2 pinin-1 mutant with 15N metabolic labeling
ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-19 | PXD030096 | Pride
Arabidopsis Acinus Quantification 15n metabolic labeling Protein prospector Targeted quantification Pinin Parallel reaction monitoring (prm) 
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