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ProteomicsDomain-specific p53 mutants activate EGFR by distinct mechanisms exposing tissue-independent therapeutic vulnerabilities
Mis-sense mutations affecting TP53 promote carcinogenesis both by inactivating its tumor suppressive functions, and by conferring aberrant pro-carcinogenic activities. We report here that mis-sense mutants in the p53 DNA-binding domain (DBD) and the transactivation domain (TAD) unexpectedly activate...
ORGANISM(S): Homo Sapiens (human) 
2023-02-17 | PXD031725 | JPOST Repository
ProteomicsMass spectrometry analysis of FLAG-5MP complexes
FLAG-tagged 5MP (h5MP1/BZW2 from human and 5MP/Kra from fly) was ectopically expressed in relevant host cells (HEK293T for human and S2 for fly) by transient transfection, and one-step affinity-purified by anti-FLAG affinity resin.The one-step affinity purification products were resolved by SDS-PAGE...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2021-05-27 | PXD026284 | JPOST Repository
GenomicsAuthentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1
Whole genome sequencing (WGS) from snap-frozen oesophageal tumour tissue and germline nucleic acids isolated from peripheral blood mononuclear cells (PBMC) was performed as part of the International Cancer Genome Consortium project and OCCAMS consortium (1,2). Filtered read sequences were mapped to ...
ORGANISM(S): Homo sapiens 
2016-09-09 | E-MTAB-4600 | biostudies-arrayexpress
TranscriptomicsMapping the cellular and molecular landscape of cardiac non-myocytes in murine type-2 diabetes
Here we define key single-cell transcriptional alterations within cardiac non-myocytes, from ventricles of spontaneously type-2 diabetic (db/db) and control (db/h) mouse hearts ([B6.BKS(D)-Lepr/J], stock #000697). The cell preparation sequenced consisted of metabolically active, live and nucleat...
ORGANISM(S): Mus musculus 
2022-11-29 | E-MTAB-11940 | biostudies-arrayexpress
TranscriptomicsTotal RNA-sequencing Detects Transcriptome Differences in Mouse Preosteoblast Response to Bulk Nanostructured Titanium
Titanium is the most widely used alloy family in dental and orthopedic implants due to its natural ability to integrate into bone, but cytotoxic alloying elements are required in titanium for its mechanical properties to match the functionality of natural bone in high-load bearing applications. Rece...
ORGANISM(S): Mus musculus 
2020-01-02 | E-MTAB-7528 | biostudies-arrayexpress
TranscriptomicsComparative proteomic and RNA-seq analyses identify glypican-3 as a hepatocyte host cell receptor for Plasmodium falciparum sporozoite invasion
Understanding the liver stage invasion and development of Plasmodium falciparum parasites is difficult due to limitations in current methodologies, namely, a hepatocyte cell line that supports the invasion of sporozoites. In this work we seek to develop and characterize a subclone of the HC-04 cell ...
ORGANISM(S): Homo sapiens 
2019-03-02 | E-MTAB-6919 | biostudies-arrayexpress
TranscriptomicsRNA-Seq of pancreatic islets from whole body knockout of lncRNA Pax6os1 mice
A whole body knockout mouse was generated by deleting the 1st exon and the 620 bp immediately upstream of the gene encoding the long non-coding RNA, Pax6os1 via CRISPR.
ORGANISM(S): Mus musculus 
2021-06-01 | E-MTAB-9213 | biostudies-arrayexpress
TranscriptomicsRNA-seq of WT and fkbp4 crispant Xenopus tadpoles
The airm of the approach was to identify genes in whole Xenopus tadpoles (Stage NF42) that are upregulated or downregulated due to disruption of the fkbp4 by CRISPR-Cas9 gene editing compared to control tadpoles. Xenopus fkbp4 crispants were generated to make a model of a novel human syndrome caused...
ORGANISM(S): Xenopus tropicalis 
2026-10-29 | E-MTAB-17717 | biostudies-arrayexpress
TranscriptomicsDeciphering the molecular immune response to COVID-19 using single cell multi-omics
Multi-omics single-cell profiling of surface proteins, gene expression and lymphocyte immune receptors from hospitalised COVID-19 patient peripheral blood immune cells and healthy controls donors. Identification of the coordinated immune cell compositional and state changes in response to SARS-CoV-2...
ORGANISM(S): Homo sapiens 
2021-03-17 | E-MTAB-10026 | biostudies-arrayexpress
GenomicsThe immunopathological landscape of human pre-TCRα deficiency: from rare to common variants
The immunopathological landscape of human pre-TCRα deficiency: from rare to common variants
PRJNA1020588 | ENA
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