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ProteomicsCoordination Between Cytoplasmic and Envelope Densities Shapes Cellular Geometry in Escherichia coli
Microbes exhibit precise control over their composition and geometry in order to adapt and grow in diverse environments. However, the mechanisms that orchestrate this simultaneous regulation, and how they are causally linked, remains poorly understood. In this work, we derive and experimentally test...
ORGANISM(S): Escherichia coli 
2025-11-26 | PXD071222 | Pride
Cell growth Cell size Proteome allocations 
ProteomicsGenome dilution by cell growth drives starvation-like proteome remodeling in yeast and mammalian cells
Cell size is tightly controlled in healthy tissues and single-celled organisms, but it remains unclear how cell size influences physiology. Increasing cell size was recently shown to remodel the proteomes of cultured human cells, demonstrating that large and small cells of the same type can be compo...
ORGANISM(S): Mus musculus (Mouse) Saccharomyces cerevisiae (Baker's yeast) 
2024-06-05 | PXD052786 | Pride
Yeast Tmt Human Silac 
ProteomicsA Fkh1/2 binding site array in the WHI5 promoter drives sub-scaling transcription
Cells typically regulate their size within a relatively tight range by coupling growth to the cell division cycle using a dedicated set of molecular mechanisms. In budding yeast, cells are born with a similar amount of the G1/S inhibitor protein Whi5 that is then diluted by growth throughout G1. As ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-03-31 | PXD075635 | Pride
Whi5 Cell size Size scaling 
ProteomicsCell size-dependent mRNA transcription drives proteome remodeling
Increasing cell size drives proteomic changes that impact cell physiology. However, the molecular basis of size-dependent proteome remodeling has remained unclear. Here, we develop an inducible Cyclin D1 expression system in human cells to generate populations of proliferating cells spanning over a ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-03 | PXD076071 | Pride
Transcription Protein turnover Cell size Size scaling 
ProteomicsPhosphoproteomic Analysis of Mec1/ATR Signaling in Response to Different Manners of Kinase Activation
The Mec1/ATR kinase coordinates multiple cellular responses to replication stress. In addition to its canonical role in activating the checkpoint kinase Rad53, Mec1 also plays checkpoint-independent roles in genome maintenance that are not well understood. Here we employed a combined genetic-phospho...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-01-14 | PXD009734 | Pride
Atr Phosphoproteomics Mec1 Dna2 Dpb11 Ddc1 
ProteomicsThe Rad53CHK1/CHK2-Spt21NPAT and Tel1ATM axes couple glucose tolerance to histone dosage and subtelomeric silencing
Checkpoint kinase signaling in low dextrose growth conditions: SILAC phosphoproteome (IMAC-enriched peptides) - Light channel = dun1 deletion yeast grown in low dextrose minimal media. Heavy channel = rad53 deletion yeast grown in low dextrose minimal media.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-07-28 | PXD020272 | Pride
Budding yeast Phosphoproteome Low dextrose 
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