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ProteomicsS. aureus COL anaerobiosis absolute protein quantification
Absolute protein quantification was applied to follow the dynamics of the cytoplasmic proteome of Staphylococcus aureus in response to long-term oxygen starvation. For 1,168 proteins, the majority of all expressed proteins, molecule numbers per cell have been determined to monitor the cellular inves...
ORGANISM(S): Staphylococcus aureus (strain COL) 
2016-07-11 | PXD000330 | Pride
Absolute protein quantification Lc-mse Anaerbiosis Synapt Lc-imse Staphylococcus aureus 
ProteomicsSystems-wide understanding of the osmotic response and metabolic rearrangement in Bacillus megaterium
For many years now, Bacillus megaterium has served as a microbial industrial strain for high-level production of recombinant proteins in the g/L-scale. Nevertheless, the impact of process-related stress has only been poorly characterized so far. Taking advantage of the recent technical developments ...
ORGANISM(S): Bacillus megaterium (strain DSM 319) 
2020-05-26 | PXD015605 | Pride
Proteomics Polyhydroxybutyrate Flux analysis Osmotic stress Proline Transcriptomics Bacillus megaterium 
ProteomicsProteome and carbon flux analysis of Pseudomonas aeruginosa clinical isolates from different infection sites
Pseudomonas aeruginosa is known as opportunistic pathogen frequently isolated from different infection sites such as burned wounds, lung and urinary tract. To shed light on the expression rates of cytoplasmic P. aeruginosa proteins, commonly expressed by eleven different clinical isolates, absolute ...
ORGANISM(S): Pseudomonas aeruginosa 
2016-03-29 | PXD002373 | Pride
Clinical isolates Gel-free and label-free proteomics 
ProteomicsDifferential view on the bile acids stress response of Clostridioides difficile
In this study, we aimed at the characterization of C. difficile’s stress response to the main four human bile acids. Although, a phenotypically description of growth differences upon challenge with different bile acids has been described (Lewis 2016, Thanissery 2017), there is no information on the ...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2019-02-12 | PXD010514 | Pride
General and specific stress response Proteomics Motility Clostridioides difficile Bile acids Differential analysis Flagella 
ProteomicsDifferential view on the bile acids stress response of Clostridioides difficile_longterm stress
In this study, we aimed at the characterization of C. difficile’s stress response to the main four human bile acids. Although, a phenotypically description of growth differences upon challenge with different bile acids has been described (Lewis 2016, Thanissery 2017), there is no information on the ...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2019-02-09 | PXD010525 | Pride
General and specific stress response Proteomics Motility Clostridioides difficile Bile acids Differential analysis Flagella 
ProteomicsCarbon source dependent reprograming of anaerobic metabolism in Staphylococcus aureus
To be a successful pathogen, S. aureus has to adapt its metabolism to the typically oxygen- and glucose-limited environment of the host. Under fermenting conditions and in the presence of glucose S. aureus uses glycolysis in order to generate ATP via substrate level phosphorylation and mainly lactic...
ORGANISM(S): Staphylococcus aureus (strain COL) 
2021-02-15 | PXD017966 | Pride
S. aureus Mass spectrometry Fermentation 
ProteomicsTerpenes a common language between fungi and bacteria
Previously, we investigated the effect of fungal VOCs on the behavior of phylogenetically different soil bacteria (Schmidt et al 2015). In these experiments we showed that VOCs emitted by several fungi can lead to phenotypical responses in bacteria, for example, by inducing a change in motility (Sch...
ORGANISM(S): Serratia plymuthica PRI-2C 
2018-07-19 | PXD004819 | Pride
Transcriptomics  proteomics  metabolomics  serratia plymuthica sp.  fusarium culmorum sp. Fungal-bacterial interactions  volatile organic compounds  terpenes 
ProteomicsDynamics of the Escherichia coli proteome in response to the deletion of the Tat pathway
In Escherichia coli, the Twin-arginine (Tat) secretion system is one of the main routes of the protein export to the periplasm. The Tat pathway secretes a set of proteins with important physiological functions. In our study, we investigated the influence of the deactivation of the Tat pathway on the...
ORGANISM(S): Escherichia coli 
2019-02-18 | PXD008803 | Pride
Escherichia coli Stress responses Tat pathway 
ProteomicsAnalysis of Escherichia coli CyDisCo proteome changes in response to the expression of scFv and the misfolded scFv
E. coli CyDisCo strain enables a high yield secretion of disulfide bond-containing proteins to the periplasm via Twin-arginine (Tat) pathway. Introducing two exogenous oxidases: the yeast sulfydryl oxidase (Erv1p) and human protein disulfide isomerase (PDI), the CyDisCo strain changes the cytoplasm ...
ORGANISM(S): Escherichia coli 
2019-02-18 | PXD010078 | Pride
Tat system Stress response Escherichia coli Proteome 
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