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ProteomicsTowards sensitive, streamlined high throughput protein thermal stability analysis for low sample input
This method development aims to enhance and streamline excisting PISA workflows by reducing pipetting steps and digestion method. With this workflow we want to provide a method with higher reproducibility and simplicity. This method combines the excisting OneTip protocol with low cell concentration ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-06 | PXD056894 | Pride
Lc-ms Onetip Method development Proteomics Pisa 
ProteomicsTowards sensitive, streamlined high throughput protein thermal stability analysis for low sample input
This method development aims to enhance and streamline excisting PISA workflows by reducing pipetting steps and digestion method. With this workflow we want to provide a method with higher reproducibility and simplicity. This method combines the excisting OneTip protocol with low cell concentration ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-06 | PXD061105 | Pride
Pisa lc-ms proteomics 
ProteomicsGlobal analysis of protein turnover dynamics in single cells
Single-cell proteomics (SCP) has advanced significantly, yet it remains largely unidimensional, focusing primarily on protein abundances. This limitation hinders our understanding of the dynamic processes occurring within individual cells, particularly protein turnover, which is crucial for cellular...
ORGANISM(S): Homo sapiens (Human) 
2025-03-28 | PXD058753 | Pride
Histone turnover Lc-ms Hff cells Single cel proteomics Pulsed silac 
ProteomicsEnhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics
We present a high-sensitivity SCP workflow named Chip-Tip, identifying >5000 proteins in individual HeLa cells
ORGANISM(S): Homo Sapiens 
PXD054944 |
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