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ProteomicsA structural mechanism for PP2A-B56alpha inhibition by CIP2A
Chemical cross-linking coupled to mass spectrometry was used to study the structure of the oncoprotein CIP2A and its interaction with the B56alpha regulatory subunit of protein phosphatase 2A. Cross-linking was performed using disuccinimidyl suberate (DSS) or a combination of pimelic dihydrazide (PD...
ORGANISM(S): Homo sapiens (Human) 
2022-10-21 | PXD020636 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  protein phosphatase 2a 
ProteomicsA systematic evaluation of nanocast metal oxide microspheres for phosphoproteomics applications
We used the nanocasting technique to prepare a range of different metal oxide microspheres that allow an objective comparison of the different materials. Using samples of different complexity, ranging from synthetic peptides to whole cell lysates, we characterized the binding behavior of these mater...
ORGANISM(S): Homo sapiens (Human) 
2017-05-17 | PXD002646 | Pride
Phosphoproteomics Human Orbitrap elite Phosphopeptide enrichment Hek293 
ProteomicsStoichiometric and structural analyses support the 48:12 model of the human pyruvate dehydrogenase complex core
Chemical cross-linking coupled to mass spectrometry using the amine-reactive BS3 reagent was used to study the organization of the human pyruvate dehydrogenase complex core consisting of multiple copies of dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase-binding protein (E3...
ORGANISM(S): Homo sapiens (Human) 
2024-05-15 | PXD045858 | Pride
Chemical cross-linking  bs3  structural proteomics  pyruvate dehydrogenase 
ProteomicsMolecular basis for RanGTP independent disassembly of an importin:ribosomal protein complex by the escortin Tsr2
Chemical cross-linking coupled to mass spectrometry was used to study the complex between the ribosomal protein, eS26A, and the escortin, Tsr2. Cross-linking was performed using different cross-linking chemistries: (1) disuccinimidyl suberate (DSS); (2) a combination of adipic acid dihydrazide (ADH)...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-09-10 | PXD009106 | Pride
Chemical cross-linking  dss  adh  pdh  dmtmm  structural proteomics  ribosome  ribosomal assembly 
ProteomicsConformational control and DNA binding mechanism of the metazoan origin recognition complex
Chemical cross-linking coupled to mass spectrometry was used to study the architecture and conformational state(s) of the Drosophila melanogaster origin replication complex (ORC). Two versions of the ORC were subjected to cross-linking with the amine-reactive reagents, disuccinimidyl suberate (DSS)....
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2018-06-11 | PXD009526 | Pride
Chemical cross-linking  dss  structural proteomics  origin recognition complex Autoinhibition 
ProteomicsThe human GID complex engages two independent modules for substrate recruitment
Chemical cross-linking coupled to mass spectrometry was used to study two different assemblies of the human E3 ubiquitin ligase complex, GID. The 5mer complex consisted of subunits MAEA, RMND5a, TWA1, WDR26, and RanBP9; the 6mer complex consisted of the subunits of the 5mer complex and the additiona...
ORGANISM(S): Homo sapiens (Human) 
2021-08-25 | PXD024822 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  gid complex 
ProteomicsCASP XL-MS target T0975: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for different homo- and heterooligomeric complexes as part of the CASP13 experiment (https://www.predictioncenter.org/casp13/index.cgi).
ORGANISM(S): Homo sapiens (Human) 
2019-10-02 | PXD010385 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  casp 
ProteomicsCASP XL-MS target H0957 (ID31): Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for different homo- and heterooligomeric complexes as part of the CASP13 experiment (https://www.predictioncenter.org/casp13/index.cgi).
ORGANISM(S): Escherichia coli 
2019-10-02 | PXD010003 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  casp 
ProteomicsCASP XL-MS target T0987: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for different homo- and heterooligomeric complexes as part of the CASP13 experiment (https://www.predictioncenter.org/casp13/index.cgi).
ORGANISM(S): Enterococcus faecalis (Streptococcus faecalis) 
2019-10-02 | PXD010410 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  casp 
ProteomicsCASP XL-MS target H0968 (ID32): Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)
Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for different homo- and heterooligomeric complexes as part of the CASP13 experiment (https://www.predictioncenter.org/casp13/index.cgi).
ORGANISM(S): Klebsiella pneumoniae 342 
2019-10-02 | PXD010004 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  casp 
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