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ProteomicsSimple, single-shot phosphoproteomic analysis of heat-stable tau
We developed a simple, antibody-free approach for single shot analysis of tau phosphorylation across the entire protein by liquid-chromatography tandem mass spectrometry (LC-MS/MS) to study age-related changes in tau phosphorylation. This methodology is species independent; thus, while initially dev...
ORGANISM(S): Rattus norvegicus (Rat) Macaca mulatta (Rhesus macaque) 
2021-11-25 | PXD027971 | Pride
Phosphoproteomics Brain Tau Heat-stable 
TranscriptomicsRNA-seq of crypt and villus cells from mouse small intestinal epithelium with an intestinal-epithelial specific deletion of LSD1 compared to wild type controls
To assess the role of LSD1 in mouse small intestinal epithelium, we isolated small intestinal crypts and villus from wild type (WT) (Villin-Cre -; Lsd1f/f) and intestinal-epithelial-specific knock-out (cKO) (Villin-Cre+; Lsd1f/f) mice. This experiment uses a new Cre strain with 100% deletion efficie...
ORGANISM(S): Mus musculus 
2023-08-02 | E-MTAB-10498 | biostudies-arrayexpress
TranscriptomicsRNA-seq of crypt and villus cells from small intestinal epithelium from mice treated with antibiotics with an intestinal-epithelial specific deletion of LSD1 compared to wild type
To assess the role of LSD1 in mouse small intestinal epithelium, we isolated small intestinal crypts and villus from wild type (WT) (Villin-Cre -; Lsd1f/f) and intestinal-epithelial-specific knock-out (cKO) (Villin-Cre+; Lsd1f/f) mice. This experiment uses a new Cre strain with 100% recombination ef...
ORGANISM(S): Mus musculus 
2023-08-02 | E-MTAB-10499 | biostudies-arrayexpress
UnknownSingle-molecule visualization of the effects of ionic strength and crowding on structure-mediated interactions in supercoiled DNA molecules.
Not available
S-EPMC6614806 | biostudies-literature
TranscriptomicsRNA-seq of murine small intestinal organoids treated with type I PRMT inhibitor MS023 against untreated controls
Murine small intestinal organoids were cultured in the presence or absence of 5µM inhibitor MS023 (PubChem CID 92136227), which targets type I protein arginine methyltransferases Prmt1, Prmt3, Prmt4, Prmt6, Prmt8. Organoids were grown in culture media containing EGF, Noggin and R-spondin (ENR), medi...
ORGANISM(S): Mus musculus 
2020-12-16 | E-MTAB-9291 | biostudies-arrayexpress
ProteomicsBioID Assay of BirA*-hARGLU1 or BirA*-hGR in Flp-In T-Rex 293 cells
ARGLU1 is a Transcriptional Coactivator and Splicing Regulator Important for Stress Hormone Signaling and Development Stress hormones bind and activate the glucocorticoid receptor (GR) in many tissues including the brain. We identified arginine and glutamate rich 1 (ARGLU1) in a screen for new modu...
ORGANISM(S): Homo sapiens (Human) 
2019-02-15 | PXD012555 | Pride
Nr3c1 Splicing Gr Arglu1 
ProteomicsARGLU1 RIME experiment in N2a cells
ARGLU1 is a Transcriptional Coactivator and Splicing Regulator Important for Stress Hormone Signaling and Development Stress hormones bind and activate the glucocorticoid receptor (GR) in many tissues including the brain. We identified arginine and glutamate rich 1 (ARGLU1) in a screen for new modu...
ORGANISM(S): Mus musculus (Mouse) 
2019-02-15 | PXD012406 | Pride
Nr3c1 Splicing Gr Arglu1 
TranscriptomicsRNA-seq of murine small intestinal organoids treated with Ep300/Crebbp inhibitor I-CBP112 against untreated controls
Murine small intestinal organoids were cultured in the presence or absence of 3µM inhibitor I-CBP112 (PubChem CID 90488984), which targets E1A Binding Protein P300 (Ep300) and Creb-binding protein (Crebbp, Cbp). Organoids were grown in culture media containing EGF, Noggin and R-spondin (ENR), media ...
ORGANISM(S): Mus musculus 
2020-12-16 | E-MTAB-9290 | biostudies-arrayexpress
TranscriptomicsRNAseq of small intestinal organoids from mice treated with the cytokines IL-13 and IL-22 for 72 hours in combination with the BMP signaling inhibitor DMH1
The intestinal epithelium is our first line of defense against infections of the gut and the plasticity in cellular differentiation of the intestinal epithelium is an important part of this response. The changes in cellular composition is driven by immune cell derived cytokines. Here we use signatur...
ORGANISM(S): Mus musculus 
2022-05-08 | E-MTAB-9185 | biostudies-arrayexpress
TranscriptomicsRNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1
To assess the role of LSD1 in mice small intestinal epithelium, small intestinal organoids were treated with an inhibitor for LSD1 (GSK-LSD1) and compared to untreated organoids. Similar to intestinal epithelium from mice with an intestinal epithelium specific LSD1-KO, paneth cells dissappear upon G...
ORGANISM(S): Mus musculus 
2020-11-16 | E-MTAB-9077 | biostudies-arrayexpress
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